1q2f
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1q2f.jpg|left|200px]] | [[Image:1q2f.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1q2f", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1q2f| PDB=1q2f | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR SOLUTION STRUCTURE OF A PEPTIDE FROM THE MDM-2 BINDING DOMAIN OF THE P53 PROTEIN THAT IS SELECTIVELY CYTOTOXIC TO CANCER CELLS''' | '''NMR SOLUTION STRUCTURE OF A PEPTIDE FROM THE MDM-2 BINDING DOMAIN OF THE P53 PROTEIN THAT IS SELECTIVELY CYTOTOXIC TO CANCER CELLS''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2F OCA]. | |
==Reference== | ==Reference== | ||
NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells., Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, Wang H, Biochemistry. 2004 Feb 24;43(7):1854-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14967026 14967026] | NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells., Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, Wang H, Biochemistry. 2004 Feb 24;43(7):1854-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14967026 14967026] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Brandt-Rauf, P W.]] | [[Category: Brandt-Rauf, P W.]] | ||
[[Category: Fine, R L.]] | [[Category: Fine, R L.]] | ||
| Line 29: | Line 25: | ||
[[Category: Rosal, R.]] | [[Category: Rosal, R.]] | ||
[[Category: Wang, H.]] | [[Category: Wang, H.]] | ||
| - | [[Category: | + | [[Category: Antitumor]] |
| - | [[Category: | + | [[Category: Mdm-2 binding domain]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: P53 protein]] |
| - | [[Category: | + | [[Category: Penetratin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:46:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:46, 3 May 2008
NMR SOLUTION STRUCTURE OF A PEPTIDE FROM THE MDM-2 BINDING DOMAIN OF THE P53 PROTEIN THAT IS SELECTIVELY CYTOTOXIC TO CANCER CELLS
Overview
We have recently found that a peptide from the mdm-2 binding domain of the p53 protein induced rapid membranolytic necrosis of a variety of different human cancer cell lines. To determine the role of solution structure in this peptide's selective and rapid tumor membrane disruptive behavior, we have performed two-dimensional NMR on a 32-residue sequence called PNC-27, in both an aqueous cytosolic-like and a mixed organic membrane-mimetic solution environment. In an aqueous milieu, PNC-27 contains three alpha-helical domains connected by loop structures, forming an S shape, and another similar structure with less helical structure. In a solution environment simulating a membrane, the helical domains found in water increase in length, forming three classes of structures, all of which form a U-shaped helix-coil-helix ensemble. In both solvent systems, this peptide forms amphipathic structures such that its hydrophobic residues coalesce on one face while the polar residues aggregate on the opposite face. The ability to form these unique structures in these two solution environments may allow the PNC-27 peptide to selectively and rapidly disrupt cancer cell membranes.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells., Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, Wang H, Biochemistry. 2004 Feb 24;43(7):1854-61. PMID:14967026 Page seeded by OCA on Sat May 3 05:46:54 2008
