1q2s

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[[Image:1q2s.gif|left|200px]]
[[Image:1q2s.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1q2s |SIZE=350|CAPTION= <scene name='initialview01'>1q2s</scene>, resolution 3.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1q2s", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=9DG:9-DEAZAGUANINE'>9DG</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=PQ1:PHOSPHORIC+ACID+MONO-[5-(2-AMINO-5-AMINOMETHYL-4-OXO-3,5-DIHYDRO-4H-PYRIDO[2,3-D]PYRIMIDIN-8-YL)-3,4-DIHYDROXY-TETRAHYDRO-FURAN-2-YLMETHYL]+ESTER'>PQ1</scene>, <scene name='pdbligand=ROB:1-DEOXY-RIBOFURANOSE-5&#39;-PHOSPHATE'>ROB</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Queuine_tRNA-ribosyltransferase Queuine tRNA-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TGT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 Zymomonas mobilis])
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-->
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|DOMAIN=
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{{STRUCTURE_1q2s| PDB=1q2s | SCENE= }}
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|RELATEDENTRY=[[1q2r|1Q2R]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2s OCA], [http://www.ebi.ac.uk/pdbsum/1q2s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q2s RCSB]</span>
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}}
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'''Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate'''
'''Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate'''
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[[Category: Liu, X.]]
[[Category: Liu, X.]]
[[Category: Xie, W.]]
[[Category: Xie, W.]]
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[[Category: covalent intermediat]]
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[[Category: Covalent intermediat]]
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[[Category: protein-rna complex]]
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[[Category: Protein-rna complex]]
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[[Category: tim barrel]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:47:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:08:01 2008''
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Revision as of 02:47, 3 May 2008

Image:1q2s.gif

Template:STRUCTURE 1q2s

Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate


Overview

Prokaryotic tRNA guanine transglycosylase (TGT) catalyzes replacement of guanine (G) by 7-aminomethyl-7-deazaguanine (PreQ1) at the wobble position of four specific tRNAs. Addition of 9-deazaguanine (9dzG) to a reaction mixture of Zymomonas mobilis TGT and an RNA substrate allowed us to trap, purify and crystallize a chemically competent covalent intermediate of the TGT-catalyzed reaction. The crystal structure of the TGT-RNA-9dzG ternary complex at a resolution of 2.9 A reveals, unexpectedly, that RNA is tethered to TGT through the side chain of Asp280. Thus, Asp280, instead of the previously proposed Asp102, acts as the nucleophile for the reaction. The RNA substrate adopts an unusual conformation, with four out of seven nucleotides in the loop region flipped out. Interactions between TGT and RNA revealed by the structure provide the molecular basis of the RNA substrate requirements by TGT. Furthermore, reaction of PreQ1 with the crystallized covalent intermediate provides insight into the necessary structural changes required for the TGT-catalyzed reaction to occur.

About this Structure

1Q2S is a Single protein structure of sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.

Reference

Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate., Xie W, Liu X, Huang RH, Nat Struct Biol. 2003 Oct;10(10):781-8. Epub 2003 Aug 31. PMID:12949492 Page seeded by OCA on Sat May 3 05:47:39 2008

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