1eb0
From Proteopedia
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| - | [[Image:1eb0.gif|left|200px]]<br /> | + | [[Image:1eb0.gif|left|200px]]<br /><applet load="1eb0" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1eb0" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1eb0, resolution 1.85Å" /> | caption="1eb0, resolution 1.85Å" /> | ||
'''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.'''<br /> | '''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EB0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1EB0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=ZN:Zn Binding Site For Chain A Symmetry Related Subunits Co ...'>ZN</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: urease accessory protein]] | [[Category: urease accessory protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:08:38 2007'' |
Revision as of 12:58, 18 December 2007
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CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.
Overview
Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the, insertion of Ni(2+) ions in the active site of urease. The x-ray structure, of the protein has been determined for two crystal forms, at 1.7 and 1.85, A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE, is composed of distinct N- and C-terminal domains, connected by a short, flexible linker. The structure reveals the topology of an elongated, homodimer, formed by interaction of the two C-terminal domains through, hydrophobic interactions. A single Zn(2+) ion bound to four conserved, His-100 residues, one from each monomer, connects two dimers resulting in, a tetrameric BpUreE known to be formed in concentrated solutions. The, Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps, obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A, large hydrophobic patch surrounding the metal ion site is surface-exposed, in the biologically relevant dimer. The BpUreE structure represents the, first for this class of proteins and suggests a possible role for UreE in, the urease nickel-center assembly.
About this Structure
1EB0 is a Single protein structure of sequence from Sporosarcina pasteurii with ZN as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602
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