1q8r
From Proteopedia
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'''Structure of E.coli RusA Holliday junction resolvase''' | '''Structure of E.coli RusA Holliday junction resolvase''' | ||
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[[Category: Sedelnikova, S E.]] | [[Category: Sedelnikova, S E.]] | ||
[[Category: Sharples, G J.]] | [[Category: Sharples, G J.]] | ||
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| - | [[Category: | + | [[Category: Recombination,hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:00:24 2008'' | |
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Revision as of 03:00, 3 May 2008
Structure of E.coli RusA Holliday junction resolvase
Overview
Holliday junction resolution performed by a variety of structure-specific endonucleases is a key step in DNA recombination and repair. It is believed that all resolvases carry out their reaction chemistries in a similar fashion, utilizing a divalent cation to facilitate the hydrolysis of the phosphodiester backbone of the DNA, but their architecture varies. To date, with the exception of bacteriophage T4 endonuclease VII, each of the known resolvase enzyme structures has been categorized into one of two families: the integrases and the nucleases. We have now determined the structure of the Escherichia coli RusA Holliday junction resolvase, which reveals a fourth structural class for these enzymes. The structure suggests that dimer formation is essential for Mg(2+) cation binding and hence catalysis and that like the other resolvases, RusA distorts its Holliday junction target upon binding. Key residues identified by mutagenesis experiments are well positioned to interact with the DNA.
About this Structure
1Q8R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold., Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ, Lloyd RG, Structure. 2003 Dec;11(12):1557-67. PMID:14656440 Page seeded by OCA on Sat May 3 06:00:24 2008
