1q9b
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1q9b.jpg|left|200px]] | [[Image:1q9b.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1q9b", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1q9b| PDB=1q9b | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION''' | '''CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION''' | ||
| Line 27: | Line 24: | ||
[[Category: Hernandez-Santoyo, A.]] | [[Category: Hernandez-Santoyo, A.]] | ||
[[Category: Rodriguez-Romero, A.]] | [[Category: Rodriguez-Romero, A.]] | ||
| - | [[Category: | + | [[Category: Agglutinin-toxin motif]] |
| - | [[Category: | + | [[Category: Allergen]] |
| - | [[Category: | + | [[Category: Lectin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:01:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:01, 3 May 2008
CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION
Overview
Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein molecule were chemically modified with BNPS-skatole (2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly, skin prick tests showed that hevein-positive patients gave no response with the modified allergen. Dot blot experiments carried out with anti-hevein mono- and polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for antibody-recognition, and demonstrated the specific cross-reactivity of other molecules containing hevein-like domains. We also report the structure of Hev b 6.02 at an extended resolution (1.5A) and compare its surface properties around Trp residues with those of similar regions in other allergens. Overall our results indicate that the central part of the protein, which comprises three aromatic and other acidic and polar residues, constitutes a conformational epitope.
About this Structure
1Q9B is a Single protein structure of sequence from Hevea brasiliensis. Full crystallographic information is available from OCA.
Reference
Insights into a conformational epitope of Hev b 6.02 (hevein)., Reyes-Lopez CA, Hernandez-Santoyo A, Pedraza-Escalona M, Mendoza G, Hernandez-Arana A, Rodriguez-Romero A, Biochem Biophys Res Commun. 2004 Jan 30;314(1):123-30. PMID:14715255 Page seeded by OCA on Sat May 3 06:01:48 2008
