1qdq

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[[Image:1qdq.jpg|left|200px]]
[[Image:1qdq.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1qdq |SIZE=350|CAPTION= <scene name='initialview01'>1qdq</scene>, resolution 2.18&Aring;
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The line below this paragraph, containing "STRUCTURE_1qdq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=074:[PROPYLAMINO-3-HYDROXY-BUTAN-1,4-DIONYL]-ISOLEUCYL-PROLINE'>074</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cathepsin_B Cathepsin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.1 3.4.22.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1qdq| PDB=1qdq | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qdq OCA], [http://www.ebi.ac.uk/pdbsum/1qdq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qdq RCSB]</span>
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}}
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'''X-RAY CRYSTAL STRUCTURE OF BOVINE CATHEPSIN B-CA074 COMPLEX'''
'''X-RAY CRYSTAL STRUCTURE OF BOVINE CATHEPSIN B-CA074 COMPLEX'''
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Yamamoto, A.]]
[[Category: Yamamoto, A.]]
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[[Category: cathepsin b]]
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[[Category: Cathepsin b]]
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[[Category: cathepsin b-specific inhibitor complex]]
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[[Category: Cathepsin b-specific inhibitor complex]]
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[[Category: papain]]
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[[Category: Papain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:10:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:19 2008''
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Revision as of 03:10, 3 May 2008

Image:1qdq.jpg

Template:STRUCTURE 1qdq

X-RAY CRYSTAL STRUCTURE OF BOVINE CATHEPSIN B-CA074 COMPLEX


Overview

The crystal structure of the bovine spleen cathepsin B (BSCB)-CA074 complex was refined to R = 0.152 using X-ray diffraction data up to 2.18 A resolution. BSCB is characterized by an extra Cys148-Cys252 disulfide bridge, as compared with rat and human CBs. Although the crystal structures of these enzymes showed similar overall folding, a difference was observed in the occluding loop, a structural element specific only to CB. Comparison of the torsion angles indicated the different flexibilities of their loop structures. The oxirane C6 atom of CA074 was covalently bonded to the Cys29 S(gamma) atom (C3-S(gamma)=1.81 A), where the S-configuration was transformed to the R-form. Concerning the oxirane carbon atom that participates in the covalent bonding with the Cys residue, an acceptable rule has been proposed. The substrate specificities at the Sn (n = 1-3) and Sn' (n=1 and 2) subsites of CB, together with the interaction features as to CA074, have been discussed in comparison with the crystal structure of the papain-CA028 (a CA074-related inhibitor) complex.

About this Structure

1QDQ is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Substrate specificity of bovine cathepsin B and its inhibition by CA074, based on crystal structure refinement of the complex., Yamamoto A, Tomoo K, Hara T, Murata M, Kitamura K, Ishida T, J Biochem. 2000 Apr;127(4):635-43. PMID:10739956 Page seeded by OCA on Sat May 3 06:10:11 2008

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