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5gow

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Revision as of 06:49, 13 May 2020

Solution structure of the complex between DP1 acidic region and TFIIH p62 PH domain

Structural highlights

5gow is a 2 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	GTF2H1, BTF2 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TF2H1_HUMAN] Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

Publication Abstract from PubMed

The heterodimeric transcription factor E2F1-DP1 plays crucial roles in coordinating gene expression during G1/S cell cycle progression. For transcriptional activation, the transactivation domain (TAD) of E2F1 is known to interact with the TATA-binding protein of TFIID and the p62 subunit of TFIIH. It is generally believed that DP1 facilitates E2F1 binding to target DNA and does not possess a TAD. Here, we show that an acidic region of DP1, whose function has remained elusive, binds to the plekstrin homology (PH) domain of p62 with higher affinity than that of E2F1 and contributes to transcriptional activation. The structure of the complex revealed that DP1 forms a twisted U-shaped, string-like conformation and binds to the surface of the PH domain by anchoring Phe403 into a pocket in the PH domain. The transcriptional activity of E2F1-DP1 was reduced when Phe403 of DP1 was mutated. These findings indicate that the acidic region of DP1 acts as a TAD by contacting TFIIH.

The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH.,Okuda M, Araki K, Ohtani K, Nishimura Y J Mol Biol. 2016 Dec 4;428(24 Pt B):4993-5006. doi: 10.1016/j.jmb.2016.11.001., Epub 2016 Nov 5. PMID:27825926^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Okuda M, Araki K, Ohtani K, Nishimura Y. The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH. J Mol Biol. 2016 Dec 4;428(24 Pt B):4993-5006. doi: 10.1016/j.jmb.2016.11.001., Epub 2016 Nov 5. PMID:27825926 doi:http://dx.doi.org/10.1016/j.jmb.2016.11.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5gow"

@@ Line 1: / Line 1: @@
 ==Solution structure of the complex between DP1 acidic region and TFIIH p62 PH domain==
-<StructureSection load='5gow' size='340' side='right' caption='[[5gow]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='5gow' size='340' side='right'caption='[[5gow]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5gow]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GOW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5gow]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GOW FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gow OCA], [http://pdbe.org/5gow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gow RCSB], [http://www.ebi.ac.uk/pdbsum/5gow PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gow ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTF2H1, BTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gow OCA], [http://pdbe.org/5gow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gow RCSB], [http://www.ebi.ac.uk/pdbsum/5gow PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gow ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Nishimura, Y]]
 [[Category: Okuda, M]]

5gow

From Proteopedia

Revision as of 06:49, 13 May 2020

Solution structure of the complex between DP1 acidic region and TFIIH p62 PH domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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