1qhw

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[[Image:1qhw.jpg|left|200px]]
[[Image:1qhw.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1qhw |SIZE=350|CAPTION= <scene name='initialview01'>1qhw</scene>, resolution 2.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1qhw", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ACP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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-->
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|DOMAIN=
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{{STRUCTURE_1qhw| PDB=1qhw | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qhw OCA], [http://www.ebi.ac.uk/pdbsum/1qhw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qhw RCSB]</span>
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}}
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'''PURPLE ACID PHOSPHATASE FROM RAT BONE'''
'''PURPLE ACID PHOSPHATASE FROM RAT BONE'''
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[[Category: Schneider, G.]]
[[Category: Schneider, G.]]
[[Category: Vihko, P.]]
[[Category: Vihko, P.]]
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[[Category: metal phosphatase]]
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[[Category: Metal phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:17:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:13:49 2008''
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Revision as of 03:17, 3 May 2008

Image:1qhw.jpg

Template:STRUCTURE 1qhw

PURPLE ACID PHOSPHATASE FROM RAT BONE


Overview

The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket.

About this Structure

1QHW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center., Lindqvist Y, Johansson E, Kaija H, Vihko P, Schneider G, J Mol Biol. 1999 Aug 6;291(1):135-47. PMID:10438611 Page seeded by OCA on Sat May 3 06:17:35 2008

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