1qj4

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[[Image:1qj4.jpg|left|200px]]
[[Image:1qj4.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1qj4 |SIZE=350|CAPTION= <scene name='initialview01'>1qj4</scene>, resolution 1.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1qj4", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CAT:Catalytic+Triad'>CAT</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Gluconate_dehydratase Gluconate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.39 4.2.1.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= HNL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3981 Hevea brasiliensis])
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-->
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|DOMAIN=
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{{STRUCTURE_1qj4| PDB=1qj4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qj4 OCA], [http://www.ebi.ac.uk/pdbsum/1qj4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qj4 RCSB]</span>
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}}
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'''HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION'''
'''HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION'''
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[[Category: Gugganig, M.]]
[[Category: Gugganig, M.]]
[[Category: Kratky, C.]]
[[Category: Kratky, C.]]
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[[Category: cyanhydrin formation]]
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[[Category: Cyanhydrin formation]]
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[[Category: cyanogenesis]]
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[[Category: Cyanogenesis]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: oxynitrilase]]
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[[Category: Oxynitrilase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:20:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:14:26 2008''
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Revision as of 03:20, 3 May 2008

Image:1qj4.jpg

Template:STRUCTURE 1qj4

HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION


Overview

The X-ray crystal structure of native hydroxynitrile lyase from Hevea brasiliensis (Hb-HNL) has been determined at 1.1 A resolution. It refined to a final R of 11.5% for all data and an Rfree of 14.4%. The favorable data-to-parameter ratio at atomic resolution made the refinement of individual anisotropic displacement parameters possible. The data also allowed a clear distinction of the alternate orientations of all histidine and the majority of asparagine and glutamine side chains. A number of hydrogen atoms, including one on the imidazole of the mechanistically important His-235, became visible as peaks in a difference electron density map. The structure revealed a discretely disordered sidechain of Ser-80, which is part of the putative catalytic triad. Analysis of the anisotropy indicated an increased mobility of residues near the entrance to the active site and within the active site.

About this Structure

1QJ4 is a Single protein structure of sequence from Hevea brasiliensis. Full crystallographic information is available from OCA.

Reference

Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis., Gruber K, Gugganig M, Wagner UG, Kratky C, Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:10494852 Page seeded by OCA on Sat May 3 06:20:14 2008

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OCA

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