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3mmn

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Revision as of 13:48, 4 May 2022

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+

Structural highlights

3mmn is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	cki1, At2g47430 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CKI1_ARATH] Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Publication Abstract from PubMed

Multistep phosphorelay (MSP) signaling mediates response to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1-5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1(RD) ) is responsible for recognition of CKI1 downstream signaling partners and specifically interacts with AHP2, AHP3, and AHP5 with different affinities. We studied the effects of Mg(2+) , the cofactor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF(3) (-) on CKI1(RD) in solution and we determined the crystal structure of free CKI1(RD) and CKI1(RD) in a complex with Mg(2+) . We found that CKI1(RD) structure shares similarities with the only known structure of plant HK, ETR1(RD) , with the main differences being in loop L3. Magnesium binding induces rearrangement of some residues around the active site of CKI1(RD) , as was determined by both X-ray crystallography and NMR spectroscopy. Collectively, these results provide initial insights into the nature of molecular mechanisms determining specificity of MSP signaling and MSP catalysis in plants.

Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.,Pekarova B, Klumpler T, Triskova O, Horak J, Jansen S, Dopitova R, Papouskova V, Nejedla E, Sklenar V, Marek J, Zidek L, Hejatko J, Janda L Plant J. 2011 May 13. doi: 10.1111/j.1365-313X.2011.04637.x. PMID:21569135^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nakamura A, Kakimoto T, Imamura A, Suzuki T, Ueguchi C, Mizuno T. Biochemical characterization of a putative cytokinin-responsive His-kinase, CKI1, from Arabidopsis thaliana. Biosci Biotechnol Biochem. 1999 Sep;63(9):1627-30. PMID:10610126
↑ Pischke MS, Jones LG, Otsuga D, Fernandez DE, Drews GN, Sussman MR. An Arabidopsis histidine kinase is essential for megagametogenesis. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15800-5. Epub 2002 Nov 8. PMID:12426401 doi:10.1073/pnas.232580499
↑ Hejatko J, Pernisova M, Eneva T, Palme K, Brzobohaty B. The putative sensor histidine kinase CKI1 is involved in female gametophyte development in Arabidopsis. Mol Genet Genomics. 2003 Jul;269(4):443-53. Epub 2003 May 28. PMID:12774227 doi:10.1007/s00438-003-0858-7
↑ Tran LS, Urao T, Qin F, Maruyama K, Kakimoto T, Shinozaki K, Yamaguchi-Shinozaki K. Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20623-8. Epub 2007 Dec 12. PMID:18077346 doi:http://dx.doi.org/10.1073/pnas.0706547105
↑ Hejatko J, Ryu H, Kim GT, Dobesova R, Choi S, Choi SM, Soucek P, Horak J, Pekarova B, Palme K, Brzobohaty B, Hwang I. The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots. Plant Cell. 2009 Jul;21(7):2008-21. doi: 10.1105/tpc.109.066696. Epub 2009 Jul, 21. PMID:19622803 doi:http://dx.doi.org/10.1105/tpc.109.066696
↑ Deng Y, Dong H, Mu J, Ren B, Zheng B, Ji Z, Yang WC, Liang Y, Zuo J. Arabidopsis histidine kinase CKI1 acts upstream of histidine phosphotransfer proteins to regulate female gametophyte development and vegetative growth. Plant Cell. 2010 Apr;22(4):1232-48. doi: 10.1105/tpc.108.065128. Epub 2010 Apr 2. PMID:20363773 doi:http://dx.doi.org/10.1105/tpc.108.065128
↑ Kakimoto T. CKI1, a histidine kinase homolog implicated in cytokinin signal transduction. Science. 1996 Nov 8;274(5289):982-5. PMID:8875940
↑ Pekarova B, Klumpler T, Triskova O, Horak J, Jansen S, Dopitova R, Papouskova V, Nejedla E, Sklenar V, Marek J, Zidek L, Hejatko J, Janda L. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. Plant J. 2011 May 13. doi: 10.1111/j.1365-313X.2011.04637.x. PMID:21569135 doi:10.1111/j.1365-313X.2011.04637.x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mmn"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+==
-<StructureSection load='3mmn' size='340' side='right' caption='[[3mmn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='3mmn' size='340' side='right'caption='[[3mmn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3mmn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MMN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3mmn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MMN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cki1, At2g47430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cki1, At2g47430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mmn OCA], [http://pdbe.org/3mmn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mmn RCSB], [http://www.ebi.ac.uk/pdbsum/3mmn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mmn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mmn OCA], [https://pdbe.org/3mmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mmn RCSB], [https://www.ebi.ac.uk/pdbsum/3mmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mmn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CKI1_ARATH CKI1_ARATH]] Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.<ref>PMID:10610126</ref> <ref>PMID:12426401</ref> <ref>PMID:12774227</ref> <ref>PMID:18077346</ref> <ref>PMID:19622803</ref> <ref>PMID:20363773</ref> <ref>PMID:8875940</ref>
+[[https://www.uniprot.org/uniprot/CKI1_ARATH CKI1_ARATH]] Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.<ref>PMID:10610126</ref> <ref>PMID:12426401</ref> <ref>PMID:12774227</ref> <ref>PMID:18077346</ref> <ref>PMID:19622803</ref> <ref>PMID:20363773</ref> <ref>PMID:8875940</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Chemotaxis protein|Chemotaxis protein]]
+*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
 *[[Receiver domain of sensor histidine kinase CKI1|Receiver domain of sensor histidine kinase CKI1]]
 == References ==
@@ Line 28: / Line 28: @@
 </StructureSection>
 [[Category: Arath]]
+[[Category: Large Structures]]
 [[Category: Dopitova, R]]
 [[Category: Hejatko, J]]

3mmn

From Proteopedia

Revision as of 13:48, 4 May 2022

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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