1qkg

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[[Image:1qkg.gif|left|200px]]
[[Image:1qkg.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1qkg |SIZE=350|CAPTION= <scene name='initialview01'>1qkg</scene>
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The line below this paragraph, containing "STRUCTURE_1qkg", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=64T:5-HYDROXY-THYMIDINE-5&#39;-MONOPHOSPHATE'>64T</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=TA3:(4S,5R)-3-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-5-METHYL-1,3-DIAZABICYCLO[2.2.0]HEXAN-2-ONE'>TA3</scene>
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1qkg| PDB=1qkg | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qkg OCA], [http://www.ebi.ac.uk/pdbsum/1qkg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qkg RCSB]</span>
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'''DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT'''
'''DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT'''
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==About this Structure==
==About this Structure==
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1QKG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKG OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKG OCA].
==Reference==
==Reference==
The Dewar photoproduct of thymidylyl(3'--&gt;5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10758155 10758155]
The Dewar photoproduct of thymidylyl(3'--&gt;5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10758155 10758155]
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[[Category: Protein complex]]
 
[[Category: Bae, S H.]]
[[Category: Bae, S H.]]
[[Category: Choi, B S.]]
[[Category: Choi, B S.]]
[[Category: Choi, Y J.]]
[[Category: Choi, Y J.]]
[[Category: Lee, J H.]]
[[Category: Lee, J H.]]
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[[Category: (6-4) adduct]]
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[[Category: Dewar product]]
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[[Category: dewar product]]
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[[Category: Dna photoproduct]]
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[[Category: dna photoproduct]]
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[[Category: Mutagenesis]]
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[[Category: mutagenesis]]
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[[Category: Nmr]]
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[[Category: nmr]]
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[[Category: Translesion replication]]
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[[Category: translesion replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:22:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:14:59 2008''
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Revision as of 03:22, 3 May 2008

Image:1qkg.gif

Template:STRUCTURE 1qkg

DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT


Overview

In contrast to the highly mutagenic pyrimidine(6-4)pyrimidone photoproduct, its Dewar valence isomer (Dewar product) has low mutagenic potential and produces a broad range of mutations [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar-lesion DNA decamer duplex. This DNA decamer duplex (DW/GA duplex) contains a mismatched base pair between the 3' T residue of the Dewar lesion (T6) and an opposed G residue (G15). The 3' T (T6) of the Dewar lesion formed stable hydrogen bonds with the opposing G15 residue. However, the helical bending and unwinding angles of the DW/GA duplex were much larger than those of a second duplex that contains the Dewar lesion and opposing A15 and A16 residues (DW/AA duplex). The DW/GA duplex showed poorer stacking interactions at the two bases of the Dewar product and at the adjacent A7 small middle dotT14 base pair than did the DW/AA duplex. These structural features imply that no thermal stability or conformational benefit is obtained by incorporating a G instead of an A opposite the 3' T of the Dewar lesion. These properties may thus facilitate the preferential incorporation of an A in accordance with the A rule during translesion replication and lead to the low frequency of 3' T-->C mutations observed at this site.

About this Structure

Full crystallographic information is available from OCA.

Reference

The Dewar photoproduct of thymidylyl(3'-->5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:10758155 Page seeded by OCA on Sat May 3 06:22:56 2008

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