Structural highlights
Function
[SYT5_HUMAN] May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).
Publication Abstract from PubMed
Synaptotagmins constitute a family of multifunctional integral membrane proteins found predominantly on vesicles in neural and endocrine tissues. 17 isoforms of synaptotagmin family in mammals have been identified, 7 isoforms among them are known to be able to bind Ca2+ via their C2 domains. This study presents the crystal structure of the first C2 domain (C2A domain) of synaptotagmin 5 complexed with Ca2+ at 1.90A resolution. Comparison of the Ca2+-binding pocket of synaptotagmin 5 C2A domain with other synaptotagmin C2 domains demonstrated that a serine residue locating at Ca2+-binding loop probably responsible to the conformational variation of Ca2+-binding pocket, and thus impacts the Ca2+-binding mechanism of C2 domain, which is verified by structural analysis of the serine mutant and Ca2+-binding assays via isothermal titration calorimetry. Alteration of Ca2+-binding mechanism might be correlated with different Ca2+ response rates of synaptotagmins, which is the basis of the functions of synaptotagmins in regulating various types of Ca2+-triggered vesicle-membrane fusion processes.
Structural analysis of Ca2+-binding pocket of synaptotagmin 5 C2A domain.,Qiu X, Ge J, Gao Y, Teng M, Niu L Int J Biol Macromol. 2016 Oct 25. pii: S0141-8130(16)32153-5. doi:, 10.1016/j.ijbiomac.2016.10.083. PMID:27793683[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Qiu X, Ge J, Gao Y, Teng M, Niu L. Structural analysis of Ca2+-binding pocket of synaptotagmin 5 C2A domain. Int J Biol Macromol. 2016 Oct 25. pii: S0141-8130(16)32153-5. doi:, 10.1016/j.ijbiomac.2016.10.083. PMID:27793683 doi:http://dx.doi.org/10.1016/j.ijbiomac.2016.10.083
