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| | ==Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginos== | | ==Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginos== |
| - | <StructureSection load='4f1r' size='340' side='right' caption='[[4f1r]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='4f1r' size='340' side='right'caption='[[4f1r]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f1r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F1R FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">crc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1r OCA], [https://pdbe.org/4f1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1r RCSB], [https://www.ebi.ac.uk/pdbsum/4f1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1r ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1r OCA], [http://pdbe.org/4f1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f1r RCSB], [http://www.ebi.ac.uk/pdbsum/4f1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1r ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q51380_PSEAI Q51380_PSEAI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dong, Y H]] | + | [[Category: Large Structures]] |
| - | [[Category: Gao, Z Q]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Wei, Y]] | + | [[Category: Dong YH]] |
| - | [[Category: Zhang, H]] | + | [[Category: Gao ZQ]] |
| - | [[Category: Catabolite repression control]] | + | [[Category: Wei Y]] |
| - | [[Category: Post-transcription regulator]] | + | [[Category: Zhang H]] |
| - | [[Category: Transcription regulator]]
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| Structural highlights
Function
Q51380_PSEAI
Publication Abstract from PubMed
The global metabolic regulator catabolite repression control (Crc) has recently been found to modulate the susceptibility to antibiotics and virulence in the opportunistic pathogen Pseudomonas aeruginosa and been suggested as a nonlethal target for novel antimicrobials. In P. aeruginosa, Crc couples with the CA motifs from the small RNA CrcZ to form a post-transcriptional regulator system and is removed from the 5'-end of the target mRNAs. In this study, we first reported the crystal structure of Crc from P. aeruginosa refined to 2.20 A. The structure showed that it consists of two halves with similar overall topology and there are 11 beta strands surrounded by 13 helices, forming a four-layered alpha/beta-sandwich. The circular dichroism spectroscopy revealed that it is thermostable in solution and shares similar characteristics to that in crystal. Comprehensive structural analysis and comparison with the homologies of Crc showed high similarity with several known nucleases and consequently may be classified into a member exodeoxyribonuclease III. However, it shows distinct substrate specificity (RNA as the preferred substrate) compared to these DNA endonucleases. Structural comparisons also revealed potential RNA recognition and binding region mainly consisting of five flexible loops. Our structure study provided the basis for the future application of Crc as a target to develop new antibiotics. (c) 2012 IUBMB Life, 65(1):50-57, 2013.
Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa.,Wei Y, Zhang H, Gao ZQ, Xu JH, Liu QS, Dong YH IUBMB Life. 2013 Jan;65(1):50-7. doi: 10.1002/iub.1103. PMID:23281037[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wei Y, Zhang H, Gao ZQ, Xu JH, Liu QS, Dong YH. Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa. IUBMB Life. 2013 Jan;65(1):50-7. doi: 10.1002/iub.1103. PMID:23281037 doi:http://dx.doi.org/10.1002/iub.1103
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