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| | ==Structure of Prenylagaramide maturation protease PagA== | | ==Structure of Prenylagaramide maturation protease PagA== |
| - | <StructureSection load='4h6w' size='340' side='right' caption='[[4h6w]], [[Resolution|resolution]] 2.45Å' scene=''> | + | <StructureSection load='4h6w' size='340' side='right'caption='[[4h6w]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4h6w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Planktothrix_agardhii_nies-596 Planktothrix agardhii nies-596]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H6W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4h6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii_NIES-596 Planktothrix agardhii NIES-596]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H6W FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h6v|4h6v]], [[4h6x|4h6x]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6w OCA], [https://pdbe.org/4h6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h6w RCSB], [https://www.ebi.ac.uk/pdbsum/4h6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6w ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pagA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=443922 Planktothrix agardhii NIES-596])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6w OCA], [http://pdbe.org/4h6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h6w RCSB], [http://www.ebi.ac.uk/pdbsum/4h6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F5B6Y7_PLAAG F5B6Y7_PLAAG] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Planktothrix agardhii nies-596]] | + | [[Category: Large Structures]] |
| - | [[Category: Agarwal, V]] | + | [[Category: Planktothrix agardhii NIES-596]] |
| - | [[Category: Nair, S K]] | + | [[Category: Agarwal V]] |
| - | [[Category: Hydrolase]]
| + | [[Category: Nair SK]] |
| - | [[Category: Protease]]
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| Structural highlights
Function
F5B6Y7_PLAAG
Publication Abstract from PubMed
Cyanobactins, a class of ribosomally encoded macrocylic natural products, are biosynthesized through the proteolytic processing and subsequent N-C macrocylization of ribosomal peptide precursors. Macrocylization occurs through a two-step process in which the first protease (PatA) removes the amino terminal flanking sequence from the precursor to yield a free N terminus of the precursor peptide, and the second protease (PatG) removes the C-terminal flanking sequence and then catalyzes the transamidation reaction to yield an N-C cyclized product. Here, we present the crystal structures of the protease domains of PatA and PatG from the patellamide cluster and of PagA from the prenylagaramide cluster. A comparative structural and biochemical analysis of the transamidating PatG protease reveals the presence of a unique structural element distinct from canonical subtilisin proteases, which may facilitate the N-C macrocylization of the peptide substrate.
Structures of cyanobactin maturation enzymes define a family of transamidating proteases.,Agarwal V, Pierce E, McIntosh J, Schmidt EW, Nair SK Chem Biol. 2012 Nov 21;19(11):1411-22. doi: 10.1016/j.chembiol.2012.09.012. PMID:23177196[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Agarwal V, Pierce E, McIntosh J, Schmidt EW, Nair SK. Structures of cyanobactin maturation enzymes define a family of transamidating proteases. Chem Biol. 2012 Nov 21;19(11):1411-22. doi: 10.1016/j.chembiol.2012.09.012. PMID:23177196 doi:http://dx.doi.org/10.1016/j.chembiol.2012.09.012
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