This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5lh5

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 12:04, 23 September 2020

High dose Thaumatin - 40-80 ms.

Structural highlights

5lh5 is a 1 chain structure with sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.

Publication Abstract from PubMed

Many biochemical processes take place on timescales ranging from femto-seconds to seconds. Accordingly, any time-resolved experiment must be matched to the speed of the structural changes of interest. Therefore, the timescale of interest defines the requirements of the X-ray source, instrumentation and data-collection strategy. In this study, a minimalistic approach for in situ crystallization is presented that requires only a few microlitres of sample solution containing a few hundred crystals. It is demonstrated that complete diffraction data sets, merged from multiple crystals, can be recorded within only a few minutes of beamtime and allow high-resolution structural information of high quality to be obtained with a temporal resolution of 40 ms. Global and site-specific radiation damage can be avoided by limiting the maximal dose per crystal to 400 kGy. Moreover, analysis of the data collected at higher doses allows the time-resolved observation of site-specific radiation damage. Therefore, our approach is well suited to observe structural changes and possibly enzymatic reactions in the low-millisecond regime.

A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution.,Schubert R, Kapis S, Gicquel Y, Bourenkov G, Schneider TR, Heymann M, Betzel C, Perbandt M IUCrJ. 2016 Oct 26;3(Pt 6):393-401. eCollection 2016 Nov 1. PMID:27840678^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schubert R, Kapis S, Gicquel Y, Bourenkov G, Schneider TR, Heymann M, Betzel C, Perbandt M. A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution. IUCrJ. 2016 Oct 26;3(Pt 6):393-401. eCollection 2016 Nov 1. PMID:27840678 doi:http://dx.doi.org/10.1107/S2052252516016304

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lh5"

@@ Line 1: / Line 1: @@
 ==High dose Thaumatin - 40-80 ms.==
-<StructureSection load='5lh5' size='340' side='right' caption='[[5lh5]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
+<StructureSection load='5lh5' size='340' side='right'caption='[[5lh5]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lh5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LH5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LH5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lh5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LH5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LH5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lh5 OCA], [http://pdbe.org/5lh5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lh5 RCSB], [http://www.ebi.ac.uk/pdbsum/5lh5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lh5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lh5 OCA], [http://pdbe.org/5lh5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lh5 RCSB], [http://www.ebi.ac.uk/pdbsum/5lh5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lh5 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Thaumatococcus daniellii]]
 [[Category: Betzel, C]]

5lh5

From Proteopedia

Revision as of 12:04, 23 September 2020

High dose Thaumatin - 40-80 ms.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox