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1qzm
From Proteopedia
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[[Image:1qzm.gif|left|200px]] | [[Image:1qzm.gif|left|200px]] | ||
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'''alpha-domain of ATPase''' | '''alpha-domain of ATPase''' | ||
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[[Category: Tropea, J E.]] | [[Category: Tropea, J E.]] | ||
[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
| - | [[Category: | + | [[Category: Aaa+ protein]] |
| - | [[Category: | + | [[Category: Atp-dependent protease]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Oligomerization domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:53:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:53, 3 May 2008
alpha-domain of ATPase
Overview
The crystal structure of the small, mostly helical alpha domain of the AAA+ module of the Escherichia coli ATP-dependent protease Lon has been solved by single isomorphous replacement combined with anomalous scattering and refined at 1.9A resolution to a crystallographic R factor of 17.9%. This domain, comprising residues 491-584, was obtained by chymotrypsin digestion of the recombinant full-length protease. The alpha domain of Lon contains four alpha helices and two parallel strands and resembles similar domains found in a variety of ATPases and helicases, including the oligomeric proteases HslVU and ClpAP. The highly conserved "sensor-2" Arg residue is located at the beginning of the third helix. Detailed comparison with the structures of 11 similar domains established the putative location of the nucleotide-binding site in this first fragment of Lon for which a crystal structure has become available.
About this Structure
1QZM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the AAA+ alpha domain of E. coli Lon protease at 1.9A resolution., Botos I, Melnikov EE, Cherry S, Khalatova AG, Rasulova FS, Tropea JE, Maurizi MR, Rotanova TV, Gustchina A, Wlodawer A, J Struct Biol. 2004 Apr-May;146(1-2):113-22. PMID:15037242 Page seeded by OCA on Sat May 3 06:53:45 2008
