5wss
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of T-Hg-T pair containing DNA duplex in the presence Ba2+== | |
| + | <StructureSection load='5wss' size='340' side='right' caption='[[5wss]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5wss]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WSS FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wsp|5wsp]], [[5wsq|5wsq]], [[5wsr|5wsr]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wss OCA], [http://pdbe.org/5wss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wss RCSB], [http://www.ebi.ac.uk/pdbsum/5wss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wss ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, we present five high-resolution crystal structures including one first-time reported C-HgII-T containing duplex, three T-HgII-T containing duplexes and one native duplex containing T-T pair without HgII Our structures suggest that both C-T and T-T pairs are flexible in interacting with the HgII ion with various binding modes including N3-HgII-N3, N4-HgII-N3, O2-HgII-N3 and N3-HgII-O4. Our studies also reveal that the overall conformations of the C-HgII-T and T-HgII-T pairs are affected by their neighboring residues via the interactions with the solvent molecules or other metal ions, such as SrII These results provide detailed insights into the interactions between HgII and nucleobases and the structural basis for the rational design of C-HgII-T or T-HgII-T containing DNA nanodevices in the future. | ||
| - | + | Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex.,Liu H, Cai C, Haruehanroengra P, Yao Q, Chen Y, Yang C, Luo Q, Wu B, Li J, Ma J, Sheng J, Gan J Nucleic Acids Res. 2016 Dec 19. pii: gkw1296. doi: 10.1093/nar/gkw1296. PMID:27998930<ref>PMID:27998930</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5wss" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gan, J H]] | ||
| + | [[Category: Liu, H H]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Dna duplex]] | ||
| + | [[Category: T-hg-t pair]] | ||
Revision as of 09:31, 10 March 2017
Crystal structure of T-Hg-T pair containing DNA duplex in the presence Ba2+
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Categories: Gan, J H | Liu, H H | Dna | Dna duplex | T-hg-t pair
