1r4g

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[[Image:1r4g.jpg|left|200px]]
[[Image:1r4g.jpg|left|200px]]
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{{Structure
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|PDB= 1r4g |SIZE=350|CAPTION= <scene name='initialview01'>1r4g</scene>
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The line below this paragraph, containing "STRUCTURE_1r4g", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>
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|GENE= P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11191 Sendai virus])
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{{STRUCTURE_1r4g| PDB=1r4g | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r4g OCA], [http://www.ebi.ac.uk/pdbsum/1r4g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r4g RCSB]</span>
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'''Solution structure of the Sendai virus protein X C-subdomain'''
'''Solution structure of the Sendai virus protein X C-subdomain'''
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[[Category: Tarbouriech, N.]]
[[Category: Tarbouriech, N.]]
[[Category: Timmins, P.]]
[[Category: Timmins, P.]]
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[[Category: three helix-bundle]]
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[[Category: Three helix-bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:03:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:05 2008''
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Revision as of 04:03, 3 May 2008

Template:STRUCTURE 1r4g

Solution structure of the Sendai virus protein X C-subdomain


Overview

The RNA-dependent RNA polymerase of the Sendai virus (SeV) consists of the large protein (L) and the phosphoprotein (P). P plays a crucial role in the enzyme by positioning L (which carries the polymerase activity) onto the matrix for transcription and replication formed by the RNA and the nucleoprotein, the N-RNA. P has a modular structure with distinct functional domains: an N-terminal domain involved in binding to N degrees (N that is not yet bound to RNA) and a C-terminal domain that carries the oligomerisation domain, the N-RNA binding domain and the L binding domain and that, combined with L, is active in transcription. Structural data have previously been obtained on the N-terminal domain and on the oligomerisation domain of P, but not yet on its N-RNA binding domain (also-called the X protein). Here we present an NMR and a small angle neutron scattering study of the SeV X protein. We show that this molecule presents two subdomains linked by an 11-residue linker, with the N-subdomain lacking a well-defined conformation. The 3D structure of the C-subdomain consists of three alpha-helices revealing an asymmetric charge distribution that may be important for binding to RNA-bound nucleoprotein. The structure of the entire C-terminal domain of P is modelled from its constituent parts in combination with small angle scattering data on this domain.

About this Structure

1R4G is a Single protein structure of sequence from Sendai virus. Full crystallographic information is available from OCA.

Reference

Structure and dynamics of the nucleocapsid-binding domain of the Sendai virus phosphoprotein in solution., Blanchard L, Tarbouriech N, Blackledge M, Timmins P, Burmeister WP, Ruigrok RW, Marion D, Virology. 2004 Feb 20;319(2):201-11. PMID:14980481 Page seeded by OCA on Sat May 3 07:03:46 2008

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