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| | ==Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa== | | ==Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa== |
| - | <StructureSection load='4j40' size='340' side='right' caption='[[4j40]], [[Resolution|resolution]] 2.99Å' scene=''> | + | <StructureSection load='4j40' size='340' side='right'caption='[[4j40]], [[Resolution|resolution]] 2.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j40]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hvb 3hvb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J40 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j40]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hvb 3hvb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J40 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hv8|3hv8]], [[3hv9|3hv9]], [[3hva|3hva]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j40 OCA], [https://pdbe.org/4j40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j40 RCSB], [https://www.ebi.ac.uk/pdbsum/4j40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j40 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fimX, PA4959 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic-guanylate-specific_phosphodiesterase Cyclic-guanylate-specific phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.52 3.1.4.52] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j40 OCA], [http://pdbe.org/4j40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j40 RCSB], [http://www.ebi.ac.uk/pdbsum/4j40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j40 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HUK6_PSEAE Q9HUK6_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cyclic-guanylate-specific phosphodiesterase]] | + | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Bae, N]] | + | [[Category: Bae N]] |
| - | [[Category: De, N]] | + | [[Category: De N]] |
| - | [[Category: Navarro, M V]] | + | [[Category: Navarro MV]] |
| - | [[Category: Sondermann, H]] | + | [[Category: Sondermann H]] |
| - | [[Category: Wang, Q]] | + | [[Category: Wang Q]] |
| - | [[Category: Biofilm]]
| + | |
| - | [[Category: C-di-gmp]]
| + | |
| - | [[Category: C-di-gmp effector]]
| + | |
| - | [[Category: Eal phosphodiesterase]]
| + | |
| - | [[Category: Hydrolase]]
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| Structural highlights
Function
Q9HUK6_PSEAE
Publication Abstract from PubMed
Bacterial pathogenesis involves social behavior including biofilm formation and swarming, processes that are regulated by the bacterially unique second messenger cyclic di-GMP (c-di-GMP). Diguanylate cyclases containing GGDEF and phosphodiesterases containing EAL domains have been identified as the enzymes controlling cellular c-di-GMP levels, yet less is known regarding signal transmission and the targets of c-di-GMP. FimX, a protein from Pseudomonas aeruginosa that governs twitching motility, belongs to a large subfamily containing both GGDEF and EAL domains. Biochemical and structural analyses reveals its function as a high-affinity receptor for c-di-GMP. A model for full-length FimX was generated combining solution scattering data and crystal structures of the degenerate GGDEF and EAL domains. Although FimX forms a dimer in solution via the N-terminal domains, a crystallographic EAL domain dimer suggests modes for the regulation of FimX by c-di-GMP binding. The results provide the structural basis for c-di-GMP sensing via degenerate phosphodiesterases.
Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX.,Navarro MV, De N, Bae N, Wang Q, Sondermann H Structure. 2009 Aug 12;17(8):1104-16. PMID:19679088[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Navarro MV, De N, Bae N, Wang Q, Sondermann H. Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX. Structure. 2009 Aug 12;17(8):1104-16. PMID:19679088 doi:10.1016/j.str.2009.06.010
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