1r4x
From Proteopedia
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'''Crystal Structure Analys of the Gamma-COPI Appendage domain''' | '''Crystal Structure Analys of the Gamma-COPI Appendage domain''' | ||
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[[Category: Owen, D J.]] | [[Category: Owen, D J.]] | ||
[[Category: Watson, P J.]] | [[Category: Watson, P J.]] | ||
| - | [[Category: | + | [[Category: Adp-ribosylation factor]] |
| - | [[Category: | + | [[Category: Appendage]] |
| - | [[Category: | + | [[Category: Beta sandwich]] |
| - | [[Category: | + | [[Category: Coatomer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:04:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:04, 3 May 2008
Crystal Structure Analys of the Gamma-COPI Appendage domain
Overview
COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, can be thought of as composed of two subcomplexes. The first consists of the beta-, gamma-, delta- and zeta-COP subunits which are distantly homologous to AP clathrin adaptor subunits. The second consists of the alpha-, beta'- and epsilon-COP subunits. Here, we present the structure of the appendage domain of gamma-COP and show that it has a similar overall fold as the alpha-appendage of AP2. Again, like the alpha-appendage the gamma-COP appendage possesses a single protein/protein interaction site on its platform subdomain. We show that in yeast this site binds to the ARFGAP Glo3p, and in mammalian gamma-COP this site binds to a Glo3p orthologue, ARFGAP2. On the basis of mutations in the yeast homologue of gamma-COP, Sec21p, a second binding site is proposed to exist on the gamma-COP appendage that interacts with the alpha,beta',epsilon COPI subcomplex.
About this Structure
1R4X is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Gamma-COP appendage domain - structure and function., Watson PJ, Frigerio G, Collins BM, Duden R, Owen DJ, Traffic. 2004 Feb;5(2):79-88. PMID:14690497 Page seeded by OCA on Sat May 3 07:04:48 2008
