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| | ==Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2== | | ==Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2== |
| - | <StructureSection load='4irv' size='340' side='right' caption='[[4irv]], [[Resolution|resolution]] 2.04Å' scene=''> | + | <StructureSection load='4irv' size='340' side='right'caption='[[4irv]], [[Resolution|resolution]] 2.04Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4irv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IRV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4irv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IRV FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cagA, cag26, cai, HP_0547 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori]), ASPP2, BBP, TP53BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4irv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4irv OCA], [https://pdbe.org/4irv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4irv RCSB], [https://www.ebi.ac.uk/pdbsum/4irv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4irv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4irv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4irv OCA], [http://pdbe.org/4irv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4irv RCSB], [http://www.ebi.ac.uk/pdbsum/4irv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4irv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CAGA_HELPY CAGA_HELPY]] May be necessary for the transcription, folding, export, or function of the cytotoxin. [[http://www.uniprot.org/uniprot/ASPP2_HUMAN ASPP2_HUMAN]] Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.<ref>PMID:11684014</ref> <ref>PMID:12694406</ref> <ref>PMID:19377511</ref> | + | [https://www.uniprot.org/uniprot/CAGA_HELPY CAGA_HELPY] May be necessary for the transcription, folding, export, or function of the cytotoxin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter pylori]] | + | [[Category: Helicobacter pylori 26695]] |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Nesic, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Stebbins, C E]] | + | [[Category: Nesic D]] |
| - | [[Category: Protein binding]] | + | [[Category: Stebbins CE]] |
| - | [[Category: Virulence factor and tumor suppressor]]
| + | |
| Structural highlights
Function
CAGA_HELPY May be necessary for the transcription, folding, export, or function of the cytotoxin.
Publication Abstract from PubMed
The Cytotoxin associated gene A (CagA) protein of Helicobacter pylori is associated with increased virulence and risk of cancer. Recent proteomic studies have demonstrated an association of CagA with the human tumor suppressor Apoptosis-stimulating Protein of p53-2 (ASPP2). We present here a genetic, biochemical, and structural analysis of CagA with ASPP2. Domain delineation of the 120-kDa CagA protein revealed a stable N-terminal subdomain that was used in a yeast two-hybrid screen that identified the proline-rich domain of ASPP2 as a host cellular target. Biochemical experiments confirm this interaction. The cocrystal structure to 2.0-A resolution of this N-terminal subdomain of CagA with a 7-kDa proline-rich sequence of ASPP2 reveals that this domain of CagA forms a highly specialized three-helix bundle, with large insertions in the loops connecting the helices. These insertions come together to form a deep binding cleft for a highly conserved 20-aa peptide of ASPP2. ASPP2 forms an extended helix in this groove of CagA, burying more than 1,000 A(2) of surface area. This interaction is disrupted in vitro and in vivo by structure-based, loss-of-contact point mutations of key residues in either CagA or ASPP2. Disruption of CagA and ASPP2 binding alters the function of ASPP2 and leads to the decreased survival of H. pylori-infected cells.
Structure of the Helicobacter pylori CagA oncoprotein bound to the human tumor suppressor ASPP2.,Nesic D, Buti L, Lu X, Stebbins CE Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1562-7. doi:, 10.1073/pnas.1320631111. Epub 2014 Jan 13. PMID:24474782[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nesic D, Buti L, Lu X, Stebbins CE. Structure of the Helicobacter pylori CagA oncoprotein bound to the human tumor suppressor ASPP2. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1562-7. doi:, 10.1073/pnas.1320631111. Epub 2014 Jan 13. PMID:24474782 doi:http://dx.doi.org/10.1073/pnas.1320631111
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