This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1r5x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1r5x.gif|left|200px]]
[[Image:1r5x.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1r5x |SIZE=350|CAPTION= <scene name='initialview01'>1r5x</scene>, resolution 2.30&Aring;
+
The line below this paragraph, containing "STRUCTURE_1r5x", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= AF2198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1r5x| PDB=1r5x | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5x OCA], [http://www.ebi.ac.uk/pdbsum/1r5x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r5x RCSB]</span>
+
-
}}
+
'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
Line 28: Line 25:
[[Category: Deshaies, R J.]]
[[Category: Deshaies, R J.]]
[[Category: Rees, D C.]]
[[Category: Rees, D C.]]
-
[[Category: csn5]]
+
[[Category: Csn5]]
-
[[Category: jab1]]
+
[[Category: Jab1]]
-
[[Category: jamm]]
+
[[Category: Jamm]]
-
[[Category: mov34]]
+
[[Category: Mov34]]
-
[[Category: mpn]]
+
[[Category: Mpn]]
-
[[Category: proteasome]]
+
[[Category: Proteasome]]
-
[[Category: rpn11]]
+
[[Category: Rpn11]]
-
[[Category: signalosome]]
+
[[Category: Signalosome]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:18 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:43 2008''
+

Revision as of 04:07, 3 May 2008

Image:1r5x.gif

Template:STRUCTURE 1r5x

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome


Overview

The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.

About this Structure

1R5X is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.

Reference

JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:14737182 Page seeded by OCA on Sat May 3 07:07:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r5x"
Personal tools