1rcx

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[[Image:1rcx.gif|left|200px]]
[[Image:1rcx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1rcx |SIZE=350|CAPTION= <scene name='initialview01'>1rcx</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1rcx", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=ACB:Active+Site'>ACB</scene>, <scene name='pdbsite=ACE:Active+Site'>ACE</scene>, <scene name='pdbsite=ACH:Active+Site'>ACH</scene>, <scene name='pdbsite=ACK:Active+Site'>ACK</scene>, <scene name='pdbsite=ACL:Active+Site'>ACL</scene>, <scene name='pdbsite=ACO:Active+Site'>ACO</scene>, <scene name='pdbsite=ACR:Active+Site'>ACR</scene> and <scene name='pdbsite=ACV:Active+Site'>ACV</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=RUB:RIBULOSE-1,5-DIPHOSPHATE'>RUB</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1rcx| PDB=1rcx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcx OCA], [http://www.ebi.ac.uk/pdbsum/1rcx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rcx RCSB]</span>
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}}
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'''NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE'''
'''NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE'''
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[[Category: Andersson, I.]]
[[Category: Andersson, I.]]
[[Category: Taylor, T C.]]
[[Category: Taylor, T C.]]
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[[Category: lyase (carbon-carbon)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:20:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:26:23 2008''
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Revision as of 04:20, 3 May 2008

Image:1rcx.gif


PDB ID 1rcx

Drag the structure with the mouse to rotate
1rcx, resolution 2.40Å ()
Ligands:
Activity: Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE


Overview

The three-dimensional structure of the complex of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco; EC 4.1.1.39) from spinach with its natural substrate ribulose 1,5-bisphosphate (RuBP) has been determined both under activating and non-activating conditions by X-ray crystallography to a resolution of 2.1 A and 2.4 A, respectively. Under activating conditions, the use of calcium instead of magnesium as the activator metal ion enabled us to trap the substrate in a stable complex for crystallographic analysis. Comparison of the structure of the activated and the non-activated RuBP complexes shows a tighter binding for the substrate in the non-activated form of the enzyme, in line with previous solution studies. In the non-activated complex, the substrate triggers isolation of the active site by inducing movements of flexible loop regions of the catalytic subunits. In contrast, in the activated complex the active site remains partly open, probably awaiting the binding of the gaseous substrate. By inspection of the structures and by comparison with other complexes of the enzyme we were able to identify a network of hydrogen bonds that stabilise a closed active site structure during crucial steps in the reaction. The present structure underlines the central role of the carbamylated lysine 201 in both activation and catalysis, and completes available structural information for our proposal on the mechanism of the enzyme.

About this Structure

1RCX is a Protein complex structure of sequences from Spinacia oleracea. The following page contains interesting information on the relation of 1RCX with [Rubisco]. Full crystallographic information is available from OCA.

Reference

The structure of the complex between rubisco and its natural substrate ribulose 1,5-bisphosphate., Taylor TC, Andersson I, J Mol Biol. 1997 Jan 31;265(4):432-44. PMID:9034362 Page seeded by OCA on Sat May 3 07:20:48 2008

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OCA

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