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| - | | + | #REDIRECT [[5vst]] This PDB entry is obsolete and replaced by 5vst |
| - | ==Crystal structure of murine CEACAM1b==
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| - | <StructureSection load='5f1d' size='340' side='right' caption='[[5f1d]], [[Resolution|resolution]] 3.10Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[5f1d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F1D FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1d OCA], [http://pdbe.org/5f1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f1d RCSB], [http://www.ebi.ac.uk/pdbsum/5f1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f1d ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Hosts and pathogens are locked in an evolutionary arms race. To infect mice, mouse hepatitis coronavirus (MHV) has evolved to recognize mouse CEACAM1a (mCEACAM1a) as its receptor. To elude MHV infections, mice may have evolved a variant allele from the Ceacam1a gene, called Ceacam1b, producing mCEACAM1b that is a much poorer MHV receptor than mCEACAM1a. Previous studies showed that sequence differences between mCEACAM1a and mCEACAM1b in a critical MHV-binding CC' loop partially account for the low receptor activity of mCEACAM1b, but detailed structural and molecular mechanisms for the differential MHV receptor activities of mCEACAM1a and mCEACAM1b remained elusive. Here we have determined the crystal structure of mCEACAM1b, and identified the structural differences and additional residue differences between mCEACAM1a and mCEACAM1b that affect MHV binding and entry. These differences include conformational alterations of the CC' loop as well as residue variations in other MHV-binding regions, including beta-strands C' and C" and loop C'C". Using pseudovirus entry and protein-protein binding assays, we show that substituting the structural and residue features from mCEACAM1b into mCEACAM1a reduced the viral receptor activity of mCEACAM1a, whereas substituting the reverse changes from mCEACAM1a into mCEACAM1b increased the viral receptor activity of mCEACAM1b. These results elucidate the detailed molecular mechanism for how mice may have kept pace in the evolutionary arms race with MHV by undergoing structural and residue changes in the MHV receptor, providing insight into this possible example of pathogen-driven evolution of a host receptor protein.
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| - | Structural and Molecular Evidence Suggesting Coronavirus-Driven Evolution of Mouse Receptor.,Peng G, Yang Y, Pasquarella JR, Xu L, Qian Z, Holmes KV, Li F J Biol Chem. 2016 Dec 29. pii: jbc.M116.764266. doi: 10.1074/jbc.M116.764266. PMID:28035001<ref>PMID:28035001</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5f1d" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Holmes, K V]]
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| - | [[Category: Li, F]]
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| - | [[Category: Pasquarella, J R]]
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| - | [[Category: Peng, G]]
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| - | [[Category: Qian, Z]]
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| - | [[Category: Xu, L]]
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| - | [[Category: Yang, Y]]
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| - | [[Category: Coronavirus]]
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| - | [[Category: Membrane protein]]
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