1rl0
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1rl0.gif|left|200px]] | [[Image:1rl0.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1rl0", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1rl0| PDB=1rl0 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30''' | '''Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30''' | ||
| Line 25: | Line 22: | ||
[[Category: Dianthus caryophyllus]] | [[Category: Dianthus caryophyllus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: RRNA N-glycosylase]] |
[[Category: Bolognesi, A.]] | [[Category: Bolognesi, A.]] | ||
[[Category: Falini, G.]] | [[Category: Falini, G.]] | ||
| Line 32: | Line 29: | ||
[[Category: Ripamonti, A.]] | [[Category: Ripamonti, A.]] | ||
[[Category: Stirpe, F.]] | [[Category: Stirpe, F.]] | ||
| - | [[Category: | + | [[Category: Alpha and beta region]] |
| - | [[Category: | + | [[Category: Mixed beta-sheet]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:37:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:37, 3 May 2008
Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30
Overview
Ribosome inactivating proteins (RIPs) are plant proteins with enzymatic activity identified as rRNA N-glycosidase (EC 3.2.2.22), which cleaves the N-glycosidic bond of a specific adenine on the ricin/sarcin region of rRNA, thus causing inhibition of protein synthesis. They also depurinate extensively DNA and other polynucleotides. The three-dimensional structure of dianthin 30, a type 1 (single-chain) RIP of Dianthus caryophyllus (leaves), is now described at 1.4 angstroms, a resolution never achieved before for any RIP. The fold typical of RIPs is conserved, despite some differences in the loop regions. The general structure comparison by superimposed alpha-carbon (249 atoms) and the sequence alignment by structure for dianthin 30 and saporin-S6 give a root mean square deviation of 0.625 angstroms. Despite the differences reported for the biological activities of the two RIPs, their structures fit quite well and both show a protein segment containing strands beta7, beta8, and beta9 shorter than other RIPs. However, the surface electrostatic potential in the active site region neatly distinguishes dianthin 30 from saporin-S6. The possible relationship between the charge distribution and the behavior of the proteins toward different substrates is discussed.
About this Structure
1RL0 is a Single protein structure of sequence from Dianthus caryophyllus. Full crystallographic information is available from OCA.
Reference
The 1.4 anstroms structure of dianthin 30 indicates a role of surface potential at the active site of type 1 ribosome inactivating proteins., Fermani S, Falini G, Ripamonti A, Polito L, Stirpe F, Bolognesi A, J Struct Biol. 2005 Feb;149(2):204-12. PMID:15681236 Page seeded by OCA on Sat May 3 07:37:35 2008
