1gpi
From Proteopedia
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| - | [[Image:1gpi.gif|left|200px]]<br /> | + | [[Image:1gpi.gif|left|200px]]<br /><applet load="1gpi" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1gpi" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1gpi, resolution 1.32Å" /> | caption="1gpi, resolution 1.32Å" /> | ||
'''CELLOBIOHYDROLASE CEL7D (CBH 58) FROM PHANEROCHAETE CHRYSOSPORIUM. CATALYTIC MODULE AT 1.32 ANG RESOLUTION'''<br /> | '''CELLOBIOHYDROLASE CEL7D (CBH 58) FROM PHANEROCHAETE CHRYSOSPORIUM. CATALYTIC MODULE AT 1.32 ANG RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GPI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] | + | 1GPI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Known structural/functional Site: <scene name='pdbsite=CAT:Nag Binding Site'>CAT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GPI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: reaction center]] | [[Category: reaction center]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:29:08 2007'' |
Revision as of 13:19, 18 December 2007
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CELLOBIOHYDROLASE CEL7D (CBH 58) FROM PHANEROCHAETE CHRYSOSPORIUM. CATALYTIC MODULE AT 1.32 ANG RESOLUTION
Overview
Cellobiohydrolase 58 (Cel7D) is the major cellulase produced by the, white-rot fungus Phanerochaete chrysosporium, constituting approximately, 10 % of the total secreted protein in liquid culture on cellulose. The, enzyme is classified into family 7 of the glycosyl hydrolases, together, with cellobiohydrolase I (Cel7A) and endoglucanase I (Cel7B) from, Trichoderma reesei. Like those enzymes, it catalyses cellulose hydrolysis, with net retention of the anomeric carbon configuration.The structure of, the catalytic module (431 residues) of Cel7D was determined at 3.0 A, resolution using the structure of Cel7A from T. reesei as a search model, in molecular replacement, and ultimately refined at 1.32 A resolution. The, core structure is a beta-sandwich composed of two large and mainly, antiparallel beta-sheets packed onto each other. A long cellulose-binding, groove is formed by loops on one face of the sandwich. The catalytic, residues are conserved and the mechanism is expected to be the same as for, other family members. The Phanerochaete Cel7D binding site is more open, than that of the T. reesei cellobiohydrolase, as a result of deletions and, other changes in the loop regions, which may explain observed differences, in catalytic properties. The binding site is not, however, as open as the, groove of the corresponding endoglucanase. A tyrosine residue at the, entrance of the tunnel may be part of an additional subsite not present in, the T. reesei cellobiohydrolase.The Cel7D structure was used to model the, products of the five other family 7 genes found in P. chrysosporium. The, results suggest that at least two of these will have differences in, specificity and possibly catalytic mechanism, thus offering some, explanation for the presence of Cel7 isozymes in this species, which are, differentially expressed in response to various growth conditions.
About this Structure
1GPI is a Single protein structure of sequence from Phanerochaete chrysosporium with NAG as ligand. Active as Cellulose 1,4-beta-cellobiosidase, with EC number 3.2.1.91 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes., Munoz IG, Ubhayasekera W, Henriksson H, Szabo I, Pettersson G, Johansson G, Mowbray SL, Stahlberg J, J Mol Biol. 2001 Dec 14;314(5):1097-111. PMID:11743726
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