1ru4

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[[Image:1ru4.gif|left|200px]]
[[Image:1ru4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ru4 |SIZE=350|CAPTION= <scene name='initialview01'>1ru4</scene>, resolution 1.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1ru4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PelL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])
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|DOMAIN=
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{{STRUCTURE_1ru4| PDB=1ru4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru4 OCA], [http://www.ebi.ac.uk/pdbsum/1ru4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ru4 RCSB]</span>
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}}
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'''Crystal structure of pectate lyase Pel9A'''
'''Crystal structure of pectate lyase Pel9A'''
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[[Category: Pickersgill, R W.]]
[[Category: Pickersgill, R W.]]
[[Category: Shevchik, V E.]]
[[Category: Shevchik, V E.]]
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[[Category: parallel beta-helix]]
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[[Category: Parallel beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:54:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:33:02 2008''
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Revision as of 04:54, 3 May 2008

Image:1ru4.gif

Template:STRUCTURE 1ru4

Crystal structure of pectate lyase Pel9A


Overview

The "family 9 polysaccharide lyase" pectate lyase L (Pel9A) from Erwinia chrysanthemi comprises a 10-coil parallel beta-helix domain with distinct structural features including an asparagine ladder and aromatic stack at novel positions within the superhelical structure. Pel9A has a single high affinity calcium-binding site strikingly similar to the "primary" calcium-binding site described previously for the family Pel1A pectate lyases, and there is strong evidence for a common second calcium ion that binds between enzyme and substrate in the "Michaelis" complex. Although the primary calcium ion binds substrate in subsite -1, it is the second calcium ion, whose binding site is formed by the coming together of enzyme and substrate, that facilitates abstraction of the C5 proton from the sacharride in subsite +1. The role of the second calcium is to withdraw electrons from the C6 carboxylate of the substrate, thereby acidifying the C5 proton facilitating its abstraction and resulting in an E1cb-like anti-beta-elimination mechanism. The active site geometries and mechanism of Pel1A and Pel9A are closely similar, but the catalytic base is a lysine in the Pel9A enzymes as opposed to an arginine in the Pel1A enzymes.

About this Structure

1RU4 is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi., Jenkins J, Shevchik VE, Hugouvieux-Cotte-Pattat N, Pickersgill RW, J Biol Chem. 2004 Mar 5;279(10):9139-45. Epub 2003 Dec 11. PMID:14670977 Page seeded by OCA on Sat May 3 07:54:39 2008

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