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1rwj
From Proteopedia
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[[Image:1rwj.gif|left|200px]] | [[Image:1rwj.gif|left|200px]] | ||
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'''c7-type three-heme cytochrome domain''' | '''c7-type three-heme cytochrome domain''' | ||
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[[Category: Salgueiro, C A.]] | [[Category: Salgueiro, C A.]] | ||
[[Category: Schiffer, M.]] | [[Category: Schiffer, M.]] | ||
| - | [[Category: | + | [[Category: Cytochrome c7]] |
| - | [[Category: | + | [[Category: Geobacter metallireducen]] |
| - | [[Category: | + | [[Category: Geobacter sulfurreducen]] |
| - | [[Category: | + | [[Category: Heme coordination in c-type cytochrome]] |
| - | [[Category: | + | [[Category: Multiheme cytochrome c]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:59:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:59, 3 May 2008
c7-type three-heme cytochrome domain
Overview
The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.
About this Structure
1RWJ is a Single protein structure of sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA.
Reference
Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162 Page seeded by OCA on Sat May 3 07:59:29 2008
