1grr
From Proteopedia
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| - | [[Image:1grr. | + | [[Image:1grr.jpg|left|200px]]<br /><applet load="1grr" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1grr" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1grr, resolution 2.90Å" /> | caption="1grr, resolution 2.90Å" /> | ||
'''CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE'''<br /> | '''CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae] with SO4 and CLC as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1GRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae] with SO4 and CLC as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=SO4:Clc Binding Site For Chain A'>SO4</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GRR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:33:41 2007'' |
Revision as of 13:23, 18 December 2007
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CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE
Overview
Streptomyces venezuelae synthesizes chloramphenicol (Cm), an inhibitor of, ribosomal peptidyl transferase activity, thereby inhibiting bacterial, growth. The producer escapes autoinhibition by its own secondary, metabolite through phosphorylation of Cm by chloramphenicol, phosphotransferase (CPT). In addition to active site binding, CPT binds, its product 3-phosphoryl-Cm, in an alternate product binding site. To, address the mechanisms of Cm tolerance of the producer, the crystal, structures of CPT were determined in complex with either the, nonchlorinated Cm (2-N-Ac-Cm) at 3.1 A resolution or the antibiotic's, immediate precursor, the p-amino analog p-NH(2)-Cm, at 2.9 A resolution., Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm binds to the secondary product binding, site.
About this Structure
1GRR is a Single protein structure of sequence from Streptomyces venezuelae with SO4 and CLC as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity., Izard T, Protein Sci. 2001 Aug;10(8):1508-13. PMID:11468347
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