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1rya
From Proteopedia
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'''Crystal Structure of the E. coli GDP-mannose mannosyl hydrolase in complex with GDP and MG''' | '''Crystal Structure of the E. coli GDP-mannose mannosyl hydrolase in complex with GDP and MG''' | ||
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[[Category: Legler, P M.]] | [[Category: Legler, P M.]] | ||
[[Category: Mildvan, A S.]] | [[Category: Mildvan, A S.]] | ||
| - | [[Category: | + | [[Category: Gdp-glucose]] |
| - | [[Category: | + | [[Category: Gdp-mannose]] |
| - | [[Category: | + | [[Category: Mannose]] |
| - | [[Category: | + | [[Category: Nudix]] |
| - | [[Category: | + | [[Category: Nudix mg-complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:03:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:03, 3 May 2008
Crystal Structure of the E. coli GDP-mannose mannosyl hydrolase in complex with GDP and MG
Overview
GDP-mannose glycosyl hydrolase (GDPMH) catalyzes the hydrolysis of GDP-mannose and GDP-glucose to GDP and sugar by substitution with inversion at C1 of the sugar. The enzyme has a modified Nudix motif and requires one divalent cation for activity. The 1.3 A X-ray structure of the GDPMH-Mg(2+)-GDP complex, together with kinetic, mutational, and NMR data, suggests a mechanism for the GDPMH reaction. Several residues and the divalent cation strongly promote the departure of the GDP leaving group, supporting a dissociative mechanism. Comparison of the GDPMH structure with that of a typical Nudix hydrolase suggests how sequence changes result in the switch of catalytic activity from P-O bond cleavage to C-O bond cleavage. Changes in the Nudix motif result in loss of binding of at least one Mg(2+) ion, and shortening of a loop by 6 residues shifts the catalytic base by approximately 10 A.
About this Structure
1RYA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of GDP-mannose glycosyl hydrolase, a Nudix enzyme that cleaves at carbon instead of phosphorus., Gabelli SB, Bianchet MA, Azurmendi HF, Xia Z, Sarawat V, Mildvan AS, Amzel LM, Structure. 2004 Jun;12(6):927-35. PMID:15274914 Page seeded by OCA on Sat May 3 08:03:24 2008
