1rz9

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[[Image:1rz9.gif|left|200px]]
[[Image:1rz9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1rz9 |SIZE=350|CAPTION= <scene name='initialview01'>1rz9</scene>, resolution 3.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1rz9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Adeno-associated virus - 5])
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-->
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|DOMAIN=
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{{STRUCTURE_1rz9| PDB=1rz9 | SCENE= }}
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|RELATEDENTRY=[[1m55|1M55]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB]</span>
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}}
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'''Crystal Structure of AAV Rep complexed with the Rep-binding sequence'''
'''Crystal Structure of AAV Rep complexed with the Rep-binding sequence'''
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[[Category: Perez, Z N.]]
[[Category: Perez, Z N.]]
[[Category: Ronning, D R.]]
[[Category: Ronning, D R.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:05:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:05 2008''
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Revision as of 05:05, 3 May 2008

Image:1rz9.gif

Template:STRUCTURE 1rz9

Crystal Structure of AAV Rep complexed with the Rep-binding sequence


Overview

Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.

About this Structure

1RZ9 is a Single protein structure of sequence from Adeno-associated virus - 5. Full crystallographic information is available from OCA.

Reference

The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:14967147 Page seeded by OCA on Sat May 3 08:05:45 2008

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