1rzt

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[[Image:1rzt.gif|left|200px]]
[[Image:1rzt.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1rzt |SIZE=350|CAPTION= <scene name='initialview01'>1rzt</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1rzt", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= POLL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1rzt| PDB=1rzt | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzt OCA], [http://www.ebi.ac.uk/pdbsum/1rzt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rzt RCSB]</span>
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}}
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'''Crystal structure of DNA polymerase lambda complexed with a two nucleotide gap DNA molecule'''
'''Crystal structure of DNA polymerase lambda complexed with a two nucleotide gap DNA molecule'''
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[[Category: Kunkel, T A.]]
[[Category: Kunkel, T A.]]
[[Category: Pedersen, L C.]]
[[Category: Pedersen, L C.]]
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[[Category: dna polymerase lambda]]
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[[Category: Dna polymerase lambda]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:07:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:21 2008''
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Revision as of 05:07, 3 May 2008

Image:1rzt.gif

Template:STRUCTURE 1rzt

Crystal structure of DNA polymerase lambda complexed with a two nucleotide gap DNA molecule


Overview

Human DNA polymerase lambda (Pol lambda) is a family X member with low frameshift fidelity that has been suggested to perform gap-filling DNA synthesis during base excision repair and during repair of broken ends with limited homology. Here, we present a 2.1 A crystal structure of the catalytic core of Pol lambda in complex with DNA containing a two nucleotide gap. Pol lambda makes limited contacts with the template strand at the polymerase active site, and superimposition with Pol beta in a ternary complex suggests a shift in the position of the DNA at the active site that is reminiscent of a deletion intermediate. Surprisingly, Pol lambda can adopt a closed conformation, even in the absence of dNTP binding. These observations have implications for the catalytic mechanism and putative DNA repair functions of Pol lambda.

About this Structure

1RZT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A structural solution for the DNA polymerase lambda-dependent repair of DNA gaps with minimal homology., Garcia-Diaz M, Bebenek K, Krahn JM, Blanco L, Kunkel TA, Pedersen LC, Mol Cell. 2004 Feb 27;13(4):561-72. PMID:14992725 Page seeded by OCA on Sat May 3 08:07:06 2008

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OCA

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