1s10

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[[Image:1s10.gif|left|200px]]
[[Image:1s10.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1s10 |SIZE=350|CAPTION= <scene name='initialview01'>1s10</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1s10", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DCP:2&#39;-DEOXYCYTIDINE-5&#39;-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= dpo4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])
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-->
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|DOMAIN=
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{{STRUCTURE_1s10| PDB=1s10 | SCENE= }}
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|RELATEDENTRY=[[1jx4|1JX4]], [[1ryr|1RYR]], [[1rys|1RYS]], [[1s0m|1S0M]], [[1s0n|1S0N]], [[1s0o|1S0O]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s10 OCA], [http://www.ebi.ac.uk/pdbsum/1s10 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s10 RCSB]</span>
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}}
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'''Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift'''
'''Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift'''
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[[Category: Woodgate, R.]]
[[Category: Woodgate, R.]]
[[Category: Yang, W.]]
[[Category: Yang, W.]]
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[[Category: abaic lesion]]
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[[Category: Abaic lesion]]
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[[Category: lesion bypass]]
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[[Category: Lesion bypass]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:09:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:48 2008''
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Revision as of 05:09, 3 May 2008

Image:1s10.gif

Template:STRUCTURE 1s10

Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift


Overview

Dpo4 from S. Solfataricus, a DinB-like Y family polymerase, efficiently replicates DNA past an abasic lesion. We have determined crystal structures of Dpo4 complexed with five different abasic site-containing DNA substrates and find that translesion synthesis is template directed with the abasic site looped out and the incoming nucleotide is opposite the base 5' to the lesion. The ensuing DNA synthesis generates a -1 frameshift when the abasic site remains extrahelical. Template realignment during primer extension is also observed, resulting in base substitutions or even +1 frameshifts. In the case of a +1 frameshift, the extra nucleotide is accommodated in the solvent-exposed minor groove. In addition, the structure of an unproductive Dpo4 ternary complex suggests that the flexible little finger domain facilitates DNA orientation and translocation during translesion synthesis.

About this Structure

1S10 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Snapshots of replication through an abasic lesion; structural basis for base substitutions and frameshifts., Ling H, Boudsocq F, Woodgate R, Yang W, Mol Cell. 2004 Mar 12;13(5):751-62. PMID:15023344 Page seeded by OCA on Sat May 3 08:09:35 2008

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OCA

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