1s1y
From Proteopedia
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'''Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds''' | '''Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds''' | ||
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[[Category: Srajer, V.]] | [[Category: Srajer, V.]] | ||
[[Category: Wulff, M.]] | [[Category: Wulff, M.]] | ||
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| - | [[Category: | + | [[Category: Time-resolved]] |
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Revision as of 05:11, 3 May 2008
Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds
Overview
We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.
About this Structure
1S1Y is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein., Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, Wulff M, Moffat K, Structure. 2004 Jun;12(6):1039-45. PMID:15274923 Page seeded by OCA on Sat May 3 08:11:55 2008
