1s94
From Proteopedia
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'''Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei''' | '''Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei''' | ||
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[[Category: Bracher, A.]] | [[Category: Bracher, A.]] | ||
[[Category: Weissenhorn, W.]] | [[Category: Weissenhorn, W.]] | ||
| - | [[Category: | + | [[Category: Structural plasticity]] |
| - | [[Category: | + | [[Category: Three helix bundle]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:26:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:26, 3 May 2008
Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei
Overview
BACKGROUND: Intracellular membrane fusion processes are mediated by the spatial and temporal control of SNARE complex assembly that results in the formation of a four-helical bundle, composed of one vesicle SNARE and three target membrane SNARE polypeptide chains. Syntaxins are essential t-SNAREs and are characterized by an N-terminal Habc domain, a flexible linker region, a coiled-coil or SNARE motif and a membrane anchor. The N-terminal Habc domain fulfills important regulatory functions while the coiled-coil motif, present in all SNAREs, is sufficient for SNARE complex formation, which is thought to drive membrane fusion. RESULTS: Here we report the crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei, s-syntaxin. Squid Habc crystallizes as a dimer and the monomer structure consists of a three-helical bundle. One molecule is strikingly similar to mammalian syntaxin 1A while the second one shows a structural deviation from the common fold in that the C-terminal part of helix C unwinds and adopts an extended conformation. CONCLUSION: Conservation of surface residues indicates that the cytosolic part of s-syntaxin can adopt an auto-inhibitory closed conformation that may bind squid neuronal Sec1, s-Sec1, in the same manner as observed in structure of the rat nSec1/syntaxin 1A complex. Furthermore, despite the overall structural similarity, the observed changes at the C-terminus of one molecule indicate structural plasticity in neuronal syntaxin. Implications of the structural conservation and the changes are discussed with respect to potential Habc domain binding partners such as Munc13, which facilitates the transition from the closed to the open conformation.
About this Structure
1S94 is a Single protein structure of sequence from Loligo pealei. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei reveals conformational plasticity at its C-terminus., Bracher A, Weissenhorn W, BMC Struct Biol. 2004 Mar 15;4:6. PMID:15113421 Page seeded by OCA on Sat May 3 08:26:06 2008
