1sb8

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[[Image:1sb8.jpg|left|200px]]
[[Image:1sb8.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1sb8 |SIZE=350|CAPTION= <scene name='initialview01'>1sb8</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1sb8", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UD2:URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE'>UD2</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_4-epimerase UDP-N-acetylglucosamine 4-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.7 5.1.3.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= wbpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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-->
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|DOMAIN=
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{{STRUCTURE_1sb8| PDB=1sb8 | SCENE= }}
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|RELATEDENTRY=[[1sb9|1SB9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sb8 OCA], [http://www.ebi.ac.uk/pdbsum/1sb8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sb8 RCSB]</span>
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}}
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'''Crystal structure of Pseudomonas aeruginosa UDP-N-acetylglucosamine 4-epimerase complexed with UDP-N-acetylgalactosamine'''
'''Crystal structure of Pseudomonas aeruginosa UDP-N-acetylglucosamine 4-epimerase complexed with UDP-N-acetylgalactosamine'''
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[[Category: Lam, J S.]]
[[Category: Lam, J S.]]
[[Category: 4-epimerase]]
[[Category: 4-epimerase]]
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[[Category: epimerase]]
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[[Category: Epimerase]]
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[[Category: gale]]
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[[Category: Gale]]
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[[Category: n-acetylgalactosamine]]
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[[Category: N-acetylgalactosamine]]
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[[Category: n-acetylglucosamine]]
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[[Category: N-acetylglucosamine]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: pseudomonas aeruginosa]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: sdr]]
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[[Category: Sdr]]
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[[Category: syk]]
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[[Category: Syk]]
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[[Category: udp]]
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[[Category: Udp]]
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[[Category: udp-galnac]]
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[[Category: Udp-galnac]]
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[[Category: udp-glc]]
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[[Category: Udp-glc]]
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[[Category: udp-glcnac]]
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[[Category: Udp-glcnac]]
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[[Category: wbpp]]
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[[Category: Wbpp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:30:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:42 2008''
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Revision as of 05:30, 3 May 2008

Image:1sb8.jpg

Template:STRUCTURE 1sb8

Crystal structure of Pseudomonas aeruginosa UDP-N-acetylglucosamine 4-epimerase complexed with UDP-N-acetylgalactosamine


Overview

The O antigen of lipopolysaccharide in Gram-negative bacteria plays a critical role in bacterium-host interactions, and for pathogenic bacteria it is a major virulence factor. In Pseudomonas aeruginosa serotype O6 one of the initial steps in O-antigen biosynthesis is catalyzed by a saccharide epimerase, WbpP. WbpP is a member of the UDP-hexose 4-epimerase family of enzymes and exists as a homo-dimer. This enzyme preferentially catalyzes the conversion between UDP-GlcNAc and UDPGalNAc above UDP-Glc and UDP-Gal, using NAD(+) as a cofactor. The crystal structures of WbpP in complex with cofactor and either UDP-Glc or UDP-GalNAc were determined at 2.5 and 2.1 A, respectively, which represents the first structural studies of a genuine UDP-GlcNAc 4-epimerase. These structures in combination with complementary mutagenesis studies suggest that the basis for the differential substrate specificity of WbpP is a consequence of the presence of a pliable solvent network in the active site. This information allows for a comprehensive analysis of the relationship between sequence and substrate specificity for UDP-hexose 4-epimerases and enables the formulation of consensus sequences that predict substrate specificity of UDP-hexose 4-epimerases yet to be biochemically characterized. Furthermore, the examination indicates that as little as one residue can dictate substrate specificity. Nonetheless, phylogenetic analysis suggests that this substrate specificity is an evolutionary and highly conserved property within UDP-hexose 4-epimerases.

About this Structure

1SB8 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of WbpP, a genuine UDP-N-acetylglucosamine 4-epimerase from Pseudomonas aeruginosa: substrate specificity in udp-hexose 4-epimerases., Ishiyama N, Creuzenet C, Lam JS, Berghuis AM, J Biol Chem. 2004 May 21;279(21):22635-42. Epub 2004 Mar 10. PMID:15016816 Page seeded by OCA on Sat May 3 08:30:15 2008

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