1si0
From Proteopedia
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'''Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation''' | '''Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation''' | ||
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[[Category: Snell, G.]] | [[Category: Snell, G.]] | ||
[[Category: Tari, L W.]] | [[Category: Tari, L W.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:43:51 2008'' | |
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Revision as of 05:43, 3 May 2008
Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation
Overview
We have determined the 1.35- and 1.45-A structures, respectively, of closed and open iron-loaded forms of Mannheimia haemolytica ferric ion-binding protein A. M. haemolytica is the causative agent in the economically important and fatal disease of cattle termed shipping fever. The periplasmic iron-binding protein of this gram-negative bacterium, which has homologous counterparts in many other pathogenic species, performs a key role in iron acquisition from mammalian host serum iron transport proteins and is essential for the survival of the pathogen within the host. The ferric (Fe(3+)) ion in the closed structure is bound by a novel asymmetric constellation of four ligands, including a synergistic carbonate anion. The open structure is ligated by three tyrosyl residues and a dynamically disordered solvent-exposed anion. Our results clearly implicate the synergistic anion as the primary mediator of global protein conformation and provide detailed insights into the molecular mechanisms of iron binding and release in the periplasm.
About this Structure
1SI0 is a Single protein structure of sequence from Mannheimia haemolytica. Full crystallographic information is available from OCA.
Reference
Structural basis for iron binding and release by a novel class of periplasmic iron-binding proteins found in gram-negative pathogens., Shouldice SR, Skene RJ, Dougan DR, Snell G, McRee DE, Schryvers AB, Tari LW, J Bacteriol. 2004 Jun;186(12):3903-10. PMID:15175304 Page seeded by OCA on Sat May 3 08:43:51 2008
