1snh
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1snh.gif|left|200px]] | [[Image:1snh.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1snh", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1snh| PDB=1snh | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer''' | '''Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. | |
==Reference== | ==Reference== | ||
NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15121904 15121904] | NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15121904 15121904] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Akutsu, H.]] | [[Category: Akutsu, H.]] | ||
[[Category: Choi, B S.]] | [[Category: Choi, B S.]] | ||
| Line 30: | Line 26: | ||
[[Category: Park, C J.]] | [[Category: Park, C J.]] | ||
[[Category: Shin, J S.]] | [[Category: Shin, J S.]] | ||
| - | [[Category: | + | [[Category: Cpd]] |
| - | [[Category: | + | [[Category: Dna]] |
| - | [[Category: | + | [[Category: G-t mismatch]] |
| - | [[Category: | + | [[Category: Major groove widening]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:55:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:55, 3 May 2008
Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer
Overview
The cis-syn cyclobutane pyrimidine dimer (CPD) is a cytotoxic, mutagenic and carcinogenic DNA photoproduct and is repaired by the nucleotide excision repair (NER) pathway in mammalian cells. The XPC-hHR23B complex as the initiator of global genomic NER binds to sites of certain kinds of DNA damage. Although CPDs are rarely recognized by the XPC-hHR23B complex, the presence of mismatched bases opposite a CPD significantly increased the binding affinity of the XPC-hHR23B complex to the CPD. In order to decipher the properties of the DNA structures that determine the binding affinity for XPC-hHR23B to DNA, we carried out structural analyses of the various types of CPDs by NMR spectroscopy. The DNA duplex which contains a single 3' T*G wobble pair in a CPD (CPD/GA duplex) induces little conformational distortion. However, severe distortion of the helical conformation occurs when a CPD contains double T*G wobble pairs (CPD/GG duplex) even though the T residues of the CPD form stable hydrogen bonds with the opposite G residues. The helical bending angle of the CPD/GG duplex was larger than those of the CPD/GA duplex and properly matched CPD/AA duplex. The fluctuation of the backbone conformation and significant changes in the widths of the major and minor grooves at the double T*G wobble paired site were also observed in the CPD/GG duplex. These structural features were also found in a duplex that contains the (6-4) adduct, which is efficiently recognized by the XPC-hHR23B complex. Thus, we suggest that the unique structural features of the DNA double helix (that is, helical bending, flexible backbone conformation, and significant changes of the major and/or minor grooves) might be important factors in determining the binding affinity of the XPC-hHR23B complex to DNA.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:15121904 Page seeded by OCA on Sat May 3 08:55:19 2008
Categories: Akutsu, H. | Choi, B S. | Ikegami, T. | Lee, J H. | Park, C J. | Shin, J S. | Cpd | Dna | G-t mismatch | Major groove widening
