5n35
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Gadolinium phased PBP2 (SSO6202) at 2.2 Ang== | |
| + | <StructureSection load='5n35' size='340' side='right' caption='[[5n35]], [[Resolution|resolution]] 2.24Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5n35]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N35 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5n41|5n41]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n35 OCA], [http://pdbe.org/5n35 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n35 RCSB], [http://www.ebi.ac.uk/pdbsum/5n35 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n35 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Since their initial characterization over 30 years ago, it has been believed that the archaeal B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit B-family replicative polymerases of eukaryotes. Here we reveal that the highly studied PolB1 from Sulfolobus solfataricus exists as a heterotrimeric complex in cell extracts. Two small subunits, PBP1 and PBP2, associate with distinct surfaces of the larger catalytic subunit and influence the enzymatic properties of the DNA polymerase. Thus, multi-subunit replicative DNA polymerase holoenzymes are present in all three domains of life. We reveal the architecture of the assembly by a combination of cross-linking coupled with mass spectrometry, X-ray crystallography and single-particle electron microscopy. The small subunits stabilize the holoenzyme assembly and the acidic tail of one small subunit mitigates the ability of the enzyme to perform strand-displacement synthesis, with important implications for lagging strand DNA synthesis. | ||
| - | + | Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme.,Yan J, Beattie TR, Rojas AL, Schermerhorn K, Gristwood T, Trinidad JC, Albers SV, Roversi P, Gardner AF, Abrescia NGA, Bell SD Nat Commun. 2017 May 2;8:15075. doi: 10.1038/ncomms15075. PMID:28462924<ref>PMID:28462924</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5n35" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Abrescia, N G]] | ||
| + | [[Category: Bell, S D]] | ||
| + | [[Category: Archaeal dna polymerase holoenzyme]] | ||
| + | [[Category: Polb1 binding protein 2]] | ||
| + | [[Category: Polymerase binding protein]] | ||
| + | [[Category: Protein binding]] | ||
Revision as of 15:40, 17 May 2017
Gadolinium phased PBP2 (SSO6202) at 2.2 Ang
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