1st8

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[[Image:1st8.gif|left|200px]]
[[Image:1st8.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1st8 |SIZE=350|CAPTION= <scene name='initialview01'>1st8</scene>, resolution 2.35&Aring;
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The line below this paragraph, containing "STRUCTURE_1st8", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= 1-feh IIa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13427 Cichorium intybus])
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-->
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|DOMAIN=
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{{STRUCTURE_1st8| PDB=1st8 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1st8 OCA], [http://www.ebi.ac.uk/pdbsum/1st8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1st8 RCSB]</span>
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}}
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'''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus'''
'''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus'''
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[[Category: Ranter, C J.De.]]
[[Category: Ranter, C J.De.]]
[[Category: Verhaest, M.]]
[[Category: Verhaest, M.]]
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[[Category: five fold beta propeller]]
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[[Category: Five fold beta propeller]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:06:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:46:40 2008''
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Revision as of 06:06, 3 May 2008

Image:1st8.gif

Template:STRUCTURE 1st8

Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus


Overview

Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32).

About this Structure

1ST8 is a Single protein structure of sequence from Cichorium intybus. Full crystallographic information is available from OCA.

Reference

X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:15659099 Page seeded by OCA on Sat May 3 09:06:47 2008

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