1svx
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1svx.gif|left|200px]] | [[Image:1svx.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1svx", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1svx| PDB=1svx | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein''' | '''Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15097997 15097997] | High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15097997 15097997] | ||
| - | [[Category: ]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 34: | Line 30: | ||
[[Category: Plueckthun, A.]] | [[Category: Plueckthun, A.]] | ||
[[Category: Stumpp, M T.]] | [[Category: Stumpp, M T.]] | ||
| - | [[Category: | + | [[Category: Ankyrin repeat protein]] |
| - | [[Category: | + | [[Category: Artificial complex]] |
| - | [[Category: | + | [[Category: Protein design]] |
| - | [[Category: | + | [[Category: Selected binder]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:12:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:12, 3 May 2008
Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein
Overview
We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. Libraries of two and three repeats, flanked by 'capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. We rapidly enriched target-specific binders with affinities in the low nanomolar range and determined the crystal structure of one of the selected AR proteins in complex with MBP at 2.3 A resolution. The interaction relies on the randomized positions of the designed AR protein and is comparable to natural, heterodimeric protein-protein interactions. Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries.
About this Structure
1SVX is a Protein complex structure of sequences from [1] and Escherichia coli. Full crystallographic information is available from OCA.
Reference
High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:15097997 Page seeded by OCA on Sat May 3 09:12:01 2008
