This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1sz9
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1sz9.gif|left|200px]] | [[Image:1sz9.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1sz9", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1sz9| PDB=1sz9 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model''' | '''The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model''' | ||
| Line 27: | Line 24: | ||
[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Meinhart, A.]] | [[Category: Meinhart, A.]] | ||
| - | [[Category: | + | [[Category: Arm repeat]] |
| - | [[Category: | + | [[Category: Pcf11]] |
| - | [[Category: | + | [[Category: Rna polymerase ii ctd interacting domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:18:16 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:18, 3 May 2008
The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model
Overview
During transcription, RNA polymerase (Pol) II synthesizes eukaryotic messenger RNA. Transcription is coupled to RNA processing by the carboxy-terminal domain (CTD) of Pol II, which consists of up to 52 repeats of the sequence Tyr 1-Ser 2-Pro 3-Thr 4-Ser 5-Pro 6-Ser 7 (refs 1, 2). After phosphorylation, the CTD binds tightly to a conserved CTD-interacting domain (CID) present in the proteins Pcf11 and Nrd1, which are essential and evolutionarily conserved factors for polyadenylation-dependent and -independent 3'-RNA processing, respectively. Here we describe the structure of a Ser 2-phosphorylated CTD peptide bound to the CID domain of Pcf11. The CTD motif Ser 2-Pro 3-Thr 4-Ser 5 forms a beta-turn that binds to a conserved groove in the CID domain. The Ser 2 phosphate group does not make direct contact with the CID domain, but may be recognized indirectly because it stabilizes the beta-turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta-spiral model of the CTD. The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner.
About this Structure
1SZ9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors., Meinhart A, Cramer P, Nature. 2004 Jul 8;430(6996):223-6. PMID:15241417 Page seeded by OCA on Sat May 3 09:18:16 2008
