1gxw
From Proteopedia
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| - | [[Image:1gxw.gif|left|200px]]<br /> | + | [[Image:1gxw.gif|left|200px]]<br /><applet load="1gxw" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1gxw" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1gxw, resolution 2.18Å" /> | caption="1gxw, resolution 2.18Å" /> | ||
'''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE'''<br /> | '''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus] with CA, ZN, SCN and VAL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] | + | 1GXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus] with CA, ZN, SCN and VAL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] Known structural/functional Site: <scene name='pdbsite=CA1:LYS Binding Site For Chain A'>CA1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thiocyanate]] | [[Category: thiocyanate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:44:27 2007'' |
Revision as of 13:34, 18 December 2007
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THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE
Overview
A new crystallization protocol for thermolysin (EC 3.4.24.27) from, Bacillus thermoproteolyticus is presented. After dissolving the protein in, the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were, obtained following the salting-in method. Crystal cell parameters are, isomorphous with those previously reported from DMSO/CsCl mixtures. The, new SCN(-) crystal structure has been analyzed. It shows the presence of, one thiocyanate ion in the catalytic site and several rearrangements in, the S(1) and S(2) subsites. These results are in agreement with the, measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly, poor in low ionic strength solutions, increases dramatically in the, presence of several neutral salts. The results reported here suggest, possible explanations for the solubility increase and for the inhibitory, effects of high SCN(-) concentrations on thermolysin activity.
About this Structure
1GXW is a Single protein structure of sequence from Bacillus thermoproteolyticus with CA, ZN, SCN and VAL as ligands. Active as Thermolysin, with EC number 3.4.24.27 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500
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Categories: Bacillus thermoproteolyticus | Single protein | Thermolysin | Gaucher, J.F. | Prange, T. | Selkti, M. | Tomas, A. | CA | SCN | VAL | ZN | Hydrolase | Metalloendopeptidase | Metalloprotease | Salting-in | Thiocyanate
