1t6c
From Proteopedia
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'''Structural characterization of the Ppx/GppA protein family: crystal structure of the Aquifex aeolicus family member''' | '''Structural characterization of the Ppx/GppA protein family: crystal structure of the Aquifex aeolicus family member''' | ||
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[[Category: Laurberg, M.]] | [[Category: Laurberg, M.]] | ||
[[Category: Liljas, A.]] | [[Category: Liljas, A.]] | ||
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| - | [[Category: | + | [[Category: Alpha/beta protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:35:09 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:35, 3 May 2008
Structural characterization of the Ppx/GppA protein family: crystal structure of the Aquifex aeolicus family member
Overview
Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GPPA family. The protein has a two-domain structure with an active site located in the interdomain cleft. Two crystal forms were investigated (type I and II) at resolutions of 1.53 and 2.15 A, respectively. This revealed a structural flexibility that has previously been described as a "butterfly-like" cleft opening around the active site in other actin-like superfamily proteins. A calcium ion is observed at the center of this region in type I crystals, substantiating that PPX/GPPA enzymes use metal ions for catalysis. Structural analysis suggests that nucleotides bind at a similar position to that seen in other members of the superfamily.
About this Structure
1T6C is a Single protein structure of sequence from Aquifex aeolicus vf5. Full crystallographic information is available from OCA.
Reference
Structural characterization of the stringent response related exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family., Kristensen O, Laurberg M, Liljas A, Kastrup JS, Gajhede M, Biochemistry. 2004 Jul 20;43(28):8894-900. PMID:15248747 Page seeded by OCA on Sat May 3 09:35:09 2008
