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| | ==Crystal structure of prostaglandin F synthase from Trypanosoma cruzi bound to NADP== | | ==Crystal structure of prostaglandin F synthase from Trypanosoma cruzi bound to NADP== |
| - | <StructureSection load='4gie' size='340' side='right' caption='[[4gie]], [[Resolution|resolution]] 1.25Å' scene=''> | + | <StructureSection load='4gie' size='340' side='right'caption='[[4gie]], [[Resolution|resolution]] 1.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4gie]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycc Trycc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GIE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GIE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4gie]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_strain_CL_Brener Trypanosoma cruzi strain CL Brener]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GIE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fzi|4fzi]], [[4f40|4f40]], [[4g5d|4g5d]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gie OCA], [https://pdbe.org/4gie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gie RCSB], [https://www.ebi.ac.uk/pdbsum/4gie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gie ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tc00.1047053511287.49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=353153 TRYCC])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-F_synthase Prostaglandin-F synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.188 1.1.1.188] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gie OCA], [http://pdbe.org/4gie PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gie RCSB], [http://www.ebi.ac.uk/pdbsum/4gie PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gie ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PGFS_TRYCC PGFS_TRYCC]] Catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. Has also aldo/ketoreductase activity towards the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde (By similarity). | + | [https://www.uniprot.org/uniprot/PGFS_TRYCC PGFS_TRYCC] Catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. Has also aldo/ketoreductase activity towards the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Prostaglandin-F synthase]] | + | [[Category: Large Structures]] |
| - | [[Category: Trycc]] | + | [[Category: Trypanosoma cruzi strain CL Brener]] |
| - | [[Category: Structural genomic]]
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| - | [[Category: Bimatoprost]]
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| - | [[Category: Chagas disease]]
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| - | [[Category: Eukaryotic pathogen]]
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| - | [[Category: Nadp+]]
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| - | [[Category: Nadph]]
| + | |
| - | [[Category: National institute of allergy and infectious disease]]
| + | |
| - | [[Category: Niaid]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Parasite]]
| + | |
| - | [[Category: Prostaglandin d2]]
| + | |
| - | [[Category: Rutin]]
| + | |
| - | [[Category: Ssgcid]]
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| - | [[Category: Trypanosomiasis]]
| + | |
| Structural highlights
Function
PGFS_TRYCC Catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. Has also aldo/ketoreductase activity towards the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde (By similarity).
Publication Abstract from PubMed
The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 A for T. cruzi PGF, 1.25 A for T. cruzi PGF with NADP, 1.6 A for L. major PGF and 1.8 A for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.,Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):609-14. doi:, 10.1107/S2053230X15006883. Epub 2015 Apr 21. PMID:25945716[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE. Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP. Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):609-14. doi:, 10.1107/S2053230X15006883. Epub 2015 Apr 21. PMID:25945716 doi:http://dx.doi.org/10.1107/S2053230X15006883
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