1t8k

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[[Image:1t8k.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1t8k", creates the "Structure Box" on the page.
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB]</span>
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'''Crystal Structure of apo acyl carrier protein from E. coli'''
'''Crystal Structure of apo acyl carrier protein from E. coli'''
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[[Category: Janson, C A.]]
[[Category: Janson, C A.]]
[[Category: Qiu, X.]]
[[Category: Qiu, X.]]
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[[Category: acp]]
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[[Category: Acp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:40:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:47 2008''
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Revision as of 06:40, 3 May 2008

Image:1t8k.jpg

Template:STRUCTURE 1t8k

Crystal Structure of apo acyl carrier protein from E. coli


Overview

A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.

About this Structure

1T8K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions., Qiu X, Janson CA, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924 Page seeded by OCA on Sat May 3 09:40:11 2008

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