1t8p

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[[Image:1t8p.gif|left|200px]]
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{{Structure
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|PDB= 1t8p |SIZE=350|CAPTION= <scene name='initialview01'>1t8p</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1t8p", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bisphosphoglycerate_mutase Bisphosphoglycerate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.4 5.4.2.4] </span>
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{{STRUCTURE_1t8p| PDB=1t8p | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8p OCA], [http://www.ebi.ac.uk/pdbsum/1t8p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t8p RCSB]</span>
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'''Crystal structure of Human erythrocyte 2,3-bisphosphoglycerate mutase'''
'''Crystal structure of Human erythrocyte 2,3-bisphosphoglycerate mutase'''
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[[Category: Wang, Y.]]
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[[Category: Wei, Z.]]
[[Category: Wei, Z.]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:40:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:54 2008''
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Revision as of 06:40, 3 May 2008

Image:1t8p.gif

Template:STRUCTURE 1t8p

Crystal structure of Human erythrocyte 2,3-bisphosphoglycerate mutase


Overview

Bisphosphoglycerate mutase is a trifunctional enzyme of which the main function is to synthesize 2,3-bisphosphoglycerate, the allosteric effector of hemoglobin. The gene coding for bisphosphoglycerate mutase from the human cDNA library was cloned and expressed in Escherichia coli. The protein crystals were obtained and diffract to 2.5 A and produced the first crystal structure of bisphosphoglycerate mutase. The model was refined to a crystallographic R-factor of 0.200 and R(free) of 0.266 with excellent stereochemistry. The enzyme remains a dimer in the crystal. The overall structure of the enzyme resembles that of the cofactor-dependent phosphoglycerate mutase except the regions of 13-21, 98-117, 127-151, and the C-terminal tail. The conformational changes in the backbone and the side chains of some residues reveal the structural basis for the different activities between phosphoglycerate mutase and bisphosphoglycerate mutase. The bisphosphoglycerate mutase-specific residue Gly-14 may cause the most important conformational changes, which makes the side chain of Glu-13 orient toward the active site. The positions of Glu-13 and Phe-22 prevent 2,3-bisphosphoglycerate from binding in the way proposed previously. In addition, the side chain of Glu-13 would affect the Glu-89 protonation ability responsible for the low mutase activity. Other structural variations, which could be connected with functional differences, are also discussed.

About this Structure

1T8P is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human bisphosphoglycerate mutase., Wang Y, Wei Z, Bian Q, Cheng Z, Wan M, Liu L, Gong W, J Biol Chem. 2004 Sep 10;279(37):39132-8. Epub 2004 Jul 16. PMID:15258155 Page seeded by OCA on Sat May 3 09:40:32 2008

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