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1t98

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[[Image:1t98.gif|left|200px]]
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{{Structure
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|GENE= MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB]</span>
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'''Crystal Structure of MukF(1-287)'''
'''Crystal Structure of MukF(1-287)'''
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[[Category: Berger, J M.]]
[[Category: Berger, J M.]]
[[Category: Fennell-Fezzie, R.]]
[[Category: Fennell-Fezzie, R.]]
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[[Category: coiled coil]]
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[[Category: Coiled coil]]
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[[Category: condensin]]
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[[Category: Condensin]]
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[[Category: domain swapped]]
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[[Category: Helix-turn helix]]
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Revision as of 06:41, 3 May 2008

Image:1t98.gif

Template:STRUCTURE 1t98

Crystal Structure of MukF(1-287)


Overview

The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.

About this Structure

1T98 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272 Page seeded by OCA on Sat May 3 09:41:46 2008

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