1t98
From Proteopedia
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'''Crystal Structure of MukF(1-287)''' | '''Crystal Structure of MukF(1-287)''' | ||
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[[Category: Berger, J M.]] | [[Category: Berger, J M.]] | ||
[[Category: Fennell-Fezzie, R.]] | [[Category: Fennell-Fezzie, R.]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
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| - | [[Category: | + | [[Category: Domain swapped]] |
| - | [[Category: | + | [[Category: Helix-turn helix]] |
| - | [[Category: | + | [[Category: Winged helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:41:46 2008'' | |
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Revision as of 06:41, 3 May 2008
Crystal Structure of MukF(1-287)
Overview
The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.
About this Structure
1T98 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272 Page seeded by OCA on Sat May 3 09:41:46 2008
