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5j4x

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Revision as of 07:29, 10 June 2020

Structure of tetrameric jacalin complexed with Gal beta-(1,3) Gal-beta-OMe

Structural highlights

5j4x is a 8 chain structure with sequence from Artocarpus integer. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	1ugw, 1jac, 1kuj, 1ws5, 1ws4, 1uh0, 1m26, 1ugx, 1uh1, 1ugy, 1ku8, 4r6n, 4r6o, 4r6p, 4r6q, 4r6r, 5j50, 5j51, 5j52
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. [LECB3_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.

Publication Abstract from PubMed

Crystal structures of jacalin in complex with GlcNAc beta-(1,3) Gal-beta-OMe and Gal beta-(1,3) Gal-beta-OMe have been determined. The binding of the ligands to jacalin is similar to that of analogous alpha-substituted disaccharides. However, the beta-substituted beta-(1,3) linked disaccharides get distorted at the anomeric center and the glycosidic linkage. The distortion results in higher internal energies of the ligands leading to lower affinity to the lectin. This confirms the possibility of using ligand distortion as a strategy for modulating binding affinity. Unlike in the case of beta-substituted monosaccharides bound to jacalin, where a larger distortion at the anomeric center was observed, smaller distortions are distributed among two centers in the structures of the two beta-substituted beta-(1,3) linked disaccharides presented here. These disaccharides, like the unsubstituted and alpha-substituted counterparts, bind jacalin with the reducing Gal at the primary binding site, indicating that the lower binding affinity of beta-substituted disaccharides is not enough to overcome the intrinsic propensity of Gal beta-(1,3) Gal-based disaccharides to bind jacalin with the reducing sugar at the primary site. (c) 2017 IUBMB Life, 69(2):72-78, 2017.

Distortion of the ligand molecule as a strategy for modulating binding affinity: Further studies involving complexes of jacalin with beta-substituted disaccharides.,Abhinav KV, Sharma K, Surolia A, Vijayan M IUBMB Life. 2017 Feb;69(2):72-78. doi: 10.1002/iub.1593. Epub 2017 Jan 23. PMID:28111895^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abhinav KV, Sharma K, Surolia A, Vijayan M. Distortion of the ligand molecule as a strategy for modulating binding affinity: Further studies involving complexes of jacalin with beta-substituted disaccharides. IUBMB Life. 2017 Feb;69(2):72-78. doi: 10.1002/iub.1593. Epub 2017 Jan 23. PMID:28111895 doi:http://dx.doi.org/10.1002/iub.1593

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5j4x"

@@ Line 1: / Line 1: @@
 ==Structure of tetrameric jacalin complexed with Gal beta-(1,3) Gal-beta-OMe==
-<StructureSection load='5j4x' size='340' side='right' caption='[[5j4x]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='5j4x' size='340' side='right'caption='[[5j4x]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j4x]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j4x]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J4X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MBG:METHYL-BETA-GALACTOSE'>MBG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MBG:METHYL-BETA-GALACTOSE'>MBG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ugw|1ugw]], [[1jac|1jac]], [[1kuj|1kuj]], [[1ws5|1ws5]], [[1ws4|1ws4]], [[1uh0|1uh0]], [[1m26|1m26]], [[1ugx|1ugx]], [[1uh1|1uh1]], [[1ugy|1ugy]], [[1ku8|1ku8]], [[4r6n|4r6n]], [[4r6o|4r6o]], [[4r6p|4r6p]], [[4r6q|4r6q]], [[4r6r|4r6r]], [[5j50|5j50]], [[5j51|5j51]], [[5j52|5j52]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4x OCA], [http://pdbe.org/5j4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4x RCSB], [http://www.ebi.ac.uk/pdbsum/5j4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4x ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4x OCA], [http://pdbe.org/5j4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4x RCSB], [http://www.ebi.ac.uk/pdbsum/5j4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4x ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Agglutinin|Agglutinin]]
+*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 == References ==
 <references/>
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Artocarpus integer]]
+[[Category: Large Structures]]
 [[Category: Abhinav, K V]]
 [[Category: Sharma, K]]

5j4x

From Proteopedia

Revision as of 07:29, 10 June 2020

Structure of tetrameric jacalin complexed with Gal beta-(1,3) Gal-beta-OMe

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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