1tap
From Proteopedia
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'''NMR SOLUTION STRUCTURE OF RECOMBINANT TICK ANTICOAGULANT PROTEIN (RTAP), A FACTOR XA INHIBITOR FROM THE TICK ORNITHODOROS MOUBATA''' | '''NMR SOLUTION STRUCTURE OF RECOMBINANT TICK ANTICOAGULANT PROTEIN (RTAP), A FACTOR XA INHIBITOR FROM THE TICK ORNITHODOROS MOUBATA''' | ||
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[[Category: Guntert, P.]] | [[Category: Guntert, P.]] | ||
[[Category: Wuthrich, K.]] | [[Category: Wuthrich, K.]] | ||
| - | [[Category: | + | [[Category: Proteinase inhibitor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:44:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:44, 3 May 2008
NMR SOLUTION STRUCTURE OF RECOMBINANT TICK ANTICOAGULANT PROTEIN (RTAP), A FACTOR XA INHIBITOR FROM THE TICK ORNITHODOROS MOUBATA
Overview
The solution structure of the recombinant tick anticoagulant protein (rTAP) was determined by 1H nuclear magnetic resonance (NMR) spectroscopy in aqueous solution at pH 3.6 and 36 degrees C. rTAP is a 60-residue protein functioning as a highly specific inhibitor of the coagulation protease factor Xa, which was originally isolated from the tick Ornithodoros moubata. Its regular secondary structure consists of a two-stranded antiparallel beta-sheet with residues 22-28 and 32-38, and an alpha-helix with residues 51-60. The relative orientation of these regular secondary structure elements has nearly identical counterparts in the bovine pancreatic trypsin inhibitor (BPTI). In contrast, the loop between the beta-sheet and the C-terminal alpha-helix as well as the N-terminal 20-residue segment preceding the beta-sheet adopt different three-dimensional folds in the two proteins. These observations are discussed with regard to the implication of different mechanisms of protease inhibition by rTAP and by Kunitz-type protein proteinase inhibitors.
About this Structure
1TAP is a Single protein structure of sequence from Ornithodoros moubata. Full crystallographic information is available from OCA.
Reference
NMR solution structure of the recombinant tick anticoagulant protein (rTAP), a factor Xa inhibitor from the tick Ornithodoros moubata., Antuch W, Guntert P, Billeter M, Hawthorne T, Grossenbacher H, Wuthrich K, FEBS Lett. 1994 Sep 26;352(2):251-7. PMID:7925983 Page seeded by OCA on Sat May 3 09:44:45 2008
